Multiplication of contaminant bacteria in urine and interpretation of delayed culture. Abstract A prospective study of the bacterial populations of non-infected urine was mounted in an attempt to define the length of delay between voiding and analysis during which culture would not give false positive results due to the multiplication of contaminant bacteria present at the time of voiding. The findings suggest that culture of urine within four hours of voiding is likely to give a true indication
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Aseptic Technique and Streak Plates Purpose The purpose of this exercise is to learn aseptic technique procedures and there importance and also to learn to isolate colonies using the streak plate technique. Introduction Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape‚ size‚ arrangement‚ and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia coli 25922 + red Proteus mirabilis 12453 + colourless Salmonella typhimurium 14028 + colourless Streptococcus faecalis 29212 - or partial Uses Acting as a visual ph indicator‚ the agar distinguishes those
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poisoning is caused by bacteria‚ such as E. coli. E. coli‚ or Escherichia coli‚ are bacteria found in the digestive systems of humans and animals. Certain strains of E. coli‚ when ingested‚ may cause a person to become very seriously ill. Most cases of food poisoning from E. Coli occur after eating raw or undercooked beef‚ or drinking unpasteurized milk. In this hypothetical case‚ I will discuss the treatment of a patient that has food poisoning from E. Coli bacteria. The first model we will be discussing
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COMPARISON BETWEEN PLACENTA AGAR AND NUTRIENT AGAR ON GROWTH OF Staphylococcus aureus AND Escherichia coli Chloe Dominique Acero Kristine Marie Gonzales Hannah Marie Hermosisima Patrisha joy Morales Joanna Keziah Ramos Group 4 BSMT3E Background of the Study Placenta is an organ characteristic of a true mammal during pregnancy‚ joining mother and offspring‚ providing endocrine secretion and selective exchange of soluble‚ but not particulate
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resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed by a bacteria to improve the lives of people. Transformation happened when a gene is transferred from one bacterium to another one on a plasmid. E. coli is the organism that genetics prefer to use because it can be easily grown and suspended
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a
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GARI ( TSI‚ LIA‚ MIO‚ Indole‚ Citrate‚ Urease‚ PAD‚ Malonate‚ Lactose‚ Glucose‚ Arabinose‚ Rhamnose‚ Inosite) easy steps to identify: *TSI: A/Ag------ Escherichia coli and Klebsiella pneumoniae A/Ag( yellow both slant and butt with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae
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