Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk INTRODUCTION: Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate‚ lipids produces fatty acids. If enzyme concentration increases‚ random collisions between the substrates and active sites of enzyme increase due to the increasing
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Investigation Aim: The aim of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The
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The history behind Auschwitz Concentration Camp Did you know that Auschwitz Concentration Camp was the largest camp to be set up by Nazi Germany? Auschwitz was the most notorious camp in Poland. “ As Nobel laureate Elie Wiesel warned years ago‚ to forget a holocaust is to kill twice.” What kind of treatment was used in the camp? Who were the leaders of this camp? Where did the people in this camp come from? Many people lost their lives to this labor camp. Auschwitz was a very brutal and harsh place
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Spectrophotometric determination of Ascorbic acid Concentration in an Unknown Solution Introduction: The naturally occurring organic compound with antioxidant properties is Ascorbic acid shortly referred as (AA). Normally the color of Ascorbic acid is white‚ but in some cases of impure samples it appears yellow in color. Ascorbic acid is solid in state. In water it dissolves well to give mildly acidic solutions. Ascorbic acid is one form ("vitamer") of vitamin C [1]. So‚ Ascorbic Acid (AA)
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Direction and Concentration Gradients Experiment 1. For each of the bags‚ identify whether the solution inside was hypertonic‚ hypotonic or isotonic in comparison to the beaker solution it was placed in. Bag #1’s solution was hypotonic compared to the solution of beaker #2. Bag #2’s solution was also hypotonic compared to the solution of beaker #2. Bag #3’s solution was isotonic compared to the solution of beaker #2 and Bag #4’s solution was hypertonic compared to the solution of beaker
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appear. Results and Conclusions 1. Contained in the following chart: • Concentrations are calculated by multiplying the concentrations by the amount of liters of that solution divided by the total volume. • -∆S2O8-2 is calculated by dividing the concentration of the S2O3-2 by the number of moles required‚ as seen in the chemical equation (2 moles) • ∆t was determined during the lab
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Objective To investigate the effect of carbon dioxide concentration on the rate of photosynthesis Introduction Photosynthesis is a process that is essential for every living organism. Organic substances‚ such as glucose‚ are made from carbon dioxide and water by light energy from the sun. The light energy is absorbed by chlorophyll and converted to chemical energy. During the process‚ oxygen is released as a by-product. The rate of photosynthesis is affected by a few of factors‚ including
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