and cocci morphology in grapelike clusters. Figure 2 is the Gram-negative stain with the safranin color and shows bacilli cellular morphology. Gram-positive sample Test Utilized Result Controls Gram Stain Gram-positive cocci Positive-S. epidermidis Negative- E. coli Hemolysis Test Full clearing of agar‚ Beta- Hemolysis Beta- S. pyogenes Alpha- S. pneumonia Gamma- E.faecalis Catalase Test Bubbles formed‚ positive Positive- S. aureus Negative- E.
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positive (i.e.‚ the suspect colony is S. aureus)‚ the serum will coagulate‚ resulting in a clot (sometimes the clot is so pronounced that the liquid will completely solidify). * If negative‚ the plasma remains liquid. The negative result may be S. epidermidis but only a more detailed identification test can confirm this‚ using biochemical tests as in API tests and BBL CRYSTAL methods. MATERIALS Sterile swab‚ oxidase strap‚ microorganisms (Pseudomonas aeruginosa‚ E. coli) METHODS A. Catalase
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Aseptic Technique & Culturing Microbes Questions A. What is the difference between a bactericidal and bacteriostatic agent? What is the difference between sterilization and disinfection? Control of growth refers to the prevention of growth of microorganisms. This control is affected in two basic ways: by killing microorganisms or by inhibiting the growth of microorganisms
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Enterococcus faecalis (Streptococcus)‚ Streptococcus lactis (Lactococcus)‚ Streptococcus mutans‚ Streptococcus pneumoniae‚ Micrococcus (Kocuria) roseus‚ Sarcina lutea (Micrococcus luteus)‚ Sporosarcina ureae‚ Staphylococcus aureus‚ Staphylococcus epidermidis‚ Staphylococcus saprophyticus‚ Rhodococcus rhodochorous. Procedure: A gram stain was performed to identify if unknown 6O is a gram positive or gram negative and determine its shape. The gram stain was performed as stated in Laboratory
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REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and
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Essential Oils Isolated from Artemisia Vulgaris Linn Of Philippine Origin: Its Antibacterial Activity Prepared by: Dr. Jennifer U. Dotado-Maderazo Lyceum of the Philippines University- Batangas College of Dentistry 2010 Introduction and Review of Literature Introduction The Philippines is known for its natural resources‚ being a tropical country‚ its supply of naturally occurring medicinal plants can be observed everywhere. Thus‚ the practice of traditional medicine has been passed
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Mango (Mangifera indica) leaves extract and Coconut Oil as an Antibacterial Ointment A Science Investigatory Project of Kristifany C. Mamba Bansud National High School-Regional Science High School for Region IV – MIMAROPA Pag-asa‚ Bansud‚ Oriental Mindoro Abstract The purpose of this study is to produce an antibacterial ointment out of Mango leaves extract and coconut oil. Young mango leaves were gathered and was chopped into small pieces. 50 mL of coconut oil was put in a frying pan. Then‚
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culture #72 was randomly selected and subjected to qualitative tests for identification. It is suggested that culture #72 is an example of Serratia marcescens and Micrococcus luteus. There were ten bacteria species possibilities: Stahylococcus epidermidis‚ Bacillus subtilis‚ Escherichia coli‚ Serratia marcescens‚ Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted
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Short in Stature Kirbys group perceives him as a selfish and controlling individual. He is someone who doesnt care about others ideas‚ but his own. Many managers lack basic training in managing people. But‚ even more importantly‚ managers lack the values‚ understanding‚ and awareness needed to interact effectively all day long with people. Skills and techniques are easier to teach‚ but values‚ beliefs‚ and attitudes are much harder to teach - and harder for managers to learn. A manager must believe
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The Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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