Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance By: Alexander Mak 7238991 Partner: Yasmin Ismail BIO2137 Section A7 Corrector: Chieu Anh Ta September 18‚ 2014 Introduction: The first lab’s primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in
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Abstract The enzyme peroxidase has been shown to break down H2O2. Enzymes are known to increase the rate at which a chemical reaction occurs. We looked at factors that affected the breakdown of hydrogen peroxide. These effects are the different temperatures and pH levels the enzymes were placed in. We found that the optimum‚ or best condition‚ temperature for the enzymes tested was about 22 degrees Celsius. The optimum pH level for the enzyme was 7. Introduction Enzymes are biochemical that catalyze
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Modifications The results from my preliminary experiment show that 100 cm3 of oxygen has been produced in the first 30 seconds.. This reaction is far too quick and will prevent me from analysing the effects different substrate concentrations have on enzymes if I decide to continue. I will therefore lower my yeast concentration to 1%. I will also measure the volume of oxygen produced every 15 seconds‚ instead of every 30 seconds. This will make my results more reliable and allow me to analyse my results
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hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result of altering the characteristics or properties of an enzyme by temperature‚ pH level‚ or substrate concentrate. As the optimal conditions of the enzyme are changed‚ bonds in the enzyme are broken and the enzyme unfolds at the tertiary and quaternary level. When this happens‚ the structure of the enzyme and active site is changed. With the change in the active site‚ the substrate
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TASK 4: Explain the structure of the ileum and the ultrastructure of the epithelium in relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions
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purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in mind‚ the
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(K.I.S.S.) this topic involves the study of: 1. ENZYMES & HOMEOSTASIS 2. TEMPERATURE REGULATION 3. INTERNAL TRANSPORT SYSTEMS 4. EXCRETION & WATER BALANCE but first‚ an introduction... Living Things are Made of Cells Homeostasis All living things are composed of microscopic units called cells. You learned in a previous topic about the structure of a cell and the functions of the organelles. DIAGRAM OF A LIVING CELL The enzymes that control all the chemical reactions in every
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How does temperature affect the rate of reaction for Lipase? As the temperature increases‚ so will the rate of enzyme reaction. However‚ as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately
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Science Coursework Trypsin Investigation Aim I am going to prove that temperature effects the rate in which the enzyme Trypsin breaks down milk substrate into amino acids. Investigation "We strongly recommend the use of Vanish Stain Removal Tablets in conjunction with a 900 spin wash at 70°C" This was found on a milk carton of a well-known drinks company‚ Nesquick. The major food group constituent of milk is‚ of course‚ milk. For my Science Investigation‚ I am going to test different temperatures
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Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular proteins‚ responsible for most of the chemical activities of living organisms. They act as catalysts‚ substances that speed up chemical reactions without being destroyed or altered during the process. Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze thousands of reactions every second. At the start of the reaction‚ there is no product‚ and the concentration is the
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