combination will result in a unique ratio‚ thus allowing us to compare unknown samples and discover their identity. The Rf value is essentially the rate the dotted sample will move in accordance to the solvent rising up the chromatography strip via capillary action. As the solvent gets sucked up to the solvent front‚ the sample dissolved into the paper will “grab” onto the moving solvent. Polarity plays a huge role on the ratio‚ as it affects the substance’s ability to migrate with the solvent. A higher
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20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that specific enzyme. The place
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Introduction Enzymes are proteins produced by living organisms to speed up the rate in which chemical reactions occur. This process can happen fast‚ slowly‚ or stop the chemical reaction all together depending on the temperature‚ pH and concentration. Catalase is one of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks
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varying temperatures are targeted towards being at 10ºC‚ 20ºC‚ 30ºC‚ 40ºC‚ and 50ºC. It is highly improbable that each trial for each of the 5 different temperatures will be the exact temperature that was targeted‚ so it’s just important that you end up having a temperature fairly close to the targeted temperatures so that the rates of reactions that you do receive are as correct as possible. The rates of reaction will be obtained using an apparatus that will guide the carbon dioxide gas being produced
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Introduction How does changing the surroundings of enzymes affect their reaction rate? The purpose of the experiment is to determine how different abiotic conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide‚ water
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Abstract The purpose of this experiment is to determine the concentration of acetic acid in vinegar by using 0.1 M HCl and NaOH solution. By performing three titrations to determine the concentration of the base‚ the concentration of the acid was determined to be 0.600 M. It was possible to determine its concentration by standardizing the sodium hydroxide solution used for the first three titrations and by using phenolphthalein to indicate its equivalence point. In conclusion‚ although there were
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Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Science 8 Observing Onion Cells ***Do not write anywhere on this piece of paper!!! It will be used again this afternoon!!!***** Instructions. On a piece of paper‚ write the title (see above)‚ the date‚ your block‚ and your name. Then copy out the problem (see below). Problem. What structures can you observe in an onion cell when using a compound light microscope? Hypothesis. On your lab report‚ below your Problem‚ write a Hypothesis that will “answer” the problem. Start your
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