Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato
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however‚ without enzymes‚ these reactions could possibly take several years to complete. An enzyme is a macromolecule‚ generally a protein‚ that lowers the activation energy of a reaction without being changed by the reaction‚ and this causes the reaction to occur much faster than usual (Campbell et al.‚ 2014). The act of speeding of a chemical reaction is called catalysis‚ and molecules that perform this are called catalyst. Enzymes act as catalysts‚ and there a many types of enzymes that perform many
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Photosynthesis Lab Kozlowski Kendal Schweiss Hour 6 Introduction: Photosynthesis is carbohydrate production using light and chlorophyll. It is a process when green plants and other organisms turn carbon dioxide and water into carbohydrates and oxygen‚ using light energy trapped by chlorophyll. The rate of photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. 2H2O + CO2 + light -> carbohydrate (CH2O) + O2 + H2O There is a spongy mesophyll
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relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes‚ it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate complex by the lock and key mechanism. It is hypothesized that a greater concentration of product is achieved through
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Chemistry Lab Write-Up Title: Paper Chromatography of Powdered Beverage Mix Purpose/Objective: The purpose is to be able to use liquid chromatography to separate dyes in a powdered beverage mix‚ calculate the Rf values for each dye‚ and rank the polarity of each dye present. Hypothesis: If we are to use the liquid chromatography to investigate the different dyes‚ then I think the different dyes will come up having different polarities and Rf values. Procedure/Method: Draw a line across
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Egg-speriment Write-up Part 1- Purpose: Why did we do the experiment? The purpose of this experiment was to understand how osmosis works and that the cell membrane is selectively permeable. Osmosis is the diffusion of water molecules through a selectively permeable membrane. Many cells cannot function without adequate water‚ so cells depend on osmosis. When a cell is selectively permeable‚ it means some substances can pass through while others can’t. Many examples of these were shown throughout
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waterBoiling chipProcedureA Separation of a Sodium Chloride and Charcoal Mixture#1 Place 4 g of the salt/charcoal mixture in a 100 mL beaker and add about 15 mL of distilled water. Stir the mixture for about two minutes to allow the salt to dissolve. #2 Set up a filter funnel with filter paper on a filter funnel stand. Filter the mixture and collect the filtrate in a 100 mL beaker as shown in Figure 1.1. #3 Wash the solid with a further 5 mL of water but do not add this to the filtrate. Note and record
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March 18‚ 2013 Spirometry Lab Write-Up A. Subject History The subject is a twenty eight year old female. She stands five feet eight inches tall and weighs approximately one hundred fifty pounds. The subject is a non-smoker and although she has never had any respiratory issues‚ asthma does tend to run in her family on the maternal side. The subject does however admit to feeling out of breathe easily when doing intense exercise for an extended period of time. B. Effect of CO2 on Breathing
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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