BRADFORD ASSAY Calculation Formulas: Absorbance of BSA = A595 nm Raw Data = Average of A595 nm of three wells ÷ 3 Example: A1+A2+A3 ÷ 3 In this case‚ 0.365+0.374+0.453 ÷ 3 = .397 Corrected Data =( (A595 nm well) – BackGround) or (Raw Data – Background) Background = negative control = Bradford Reagent + No Protein Background of this standard curve = A595 nm of well ‘A’ = .397 We used well ‘A’ as our negative control. DATA RAW DATA
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Yeast Lab Report Guidelines 1. Lab reports are to be computer-generated and double-spaced. All sections of the report must be written in paragraph form. 2. Do not use encyclopedias (Internet or otherwise)‚ dictionaries ((Internet or otherwise)‚ or personal web pages as sources for the report‚ this includes Wikipedia. You may use a textbook‚ lab manual‚ and/or article(s) in a published journal. You can find journal articles by going to the library website: http://www.lib.clemson.edu/ and selecting
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Physics Lab Report Format General Remarks: Writing a lab report is the only way your TA will know what you have done during the lab and how well you have understood the process and the results. Part of your lab experience should be learning how to organize and present your work in a scientific way. There is no framework that can be used as a “one size fits all”‚ therefore this sample lab report should only be used as an example. Any lab report should have the following features:
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60 seconds. The colour change was compared with the colour chart on the Uristix® bottle. The detection of glucose in urine is based on the presence of two enzymes‚ glucose oxidase and peroxidase. The first enzyme‚ glucose oxidase‚ catalyses the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. The second enzyme‚ peroxidase‚ catalyses the
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Engineering Laboratory (Lab) Report Department of Engineering Faculty of Engineering and Science‚ University Tunku Abdul Rahman (UTAR) 1. Introduction Laboratory (Lab) reports are the most frequent document written by engineering student. These reports can contribute a significant amount of marks and yet little time or attention is devoted in understanding on how to write them well. The aim of this document is to provide a general guideline on writing a lab report. A lab report should not be
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Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
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later! Protein Portion 1. OK‚ so remember that back in the day‚ we had protein and nucleic acid resulting from a centrifugation…Well‚ now we’re dealing with the protein portion‚ which is solid 1. We take half the protein and add pancreatic enzyme a. This enyzme will hydrolyze the protein into its amino acid subunits a. This simulates how the hydrolytic process is carried out naturally‚ because in real life it is done with enyzmes! 1. And the other half of the protein
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May 1‚ 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon‚ called substrates‚ often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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indicate when the equivalence point is reached as it is titrated with Fe2+ Iron is the most abundant metal in the Earth’s crust after Aluminum. It is found in many ores such as hematite‚ siderite and magnetite.Iron is a basic part of many proteins and enzymes that maintain good health. In humans‚ iron is a vital constituent of proteins involved in oxygen transport. It is also essential for the regulation of cell growth and differentiation. A deficiency of iron limits oxygen delivery to cells‚ resulting
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