1. Identify the functional group or molecule for each of the following. (3 points) 1. COOH -OH 2. COH -OH 3. COH -OH 1. List whether each of the following substances was positive or negative for reducing sugar‚ as indicated by the Benedict’s test. (6 points) 1. corn syrup (1 point) positive 2. table sugar (1 point) positive 3. unknown 1 (1 point) negative 4. unknown 2 (1 point) negative 5. unknown 3 (1 point) positive 6. unknown 4 (1 point)
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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activity of enzymes.(www.biology.kenyon.edu) Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells.(www.livescience.com) Enzymes speed up chemical reactions by providing an alternate reaction pathway and lowering activation energy. Enzymes are vital for life and serve a wide range of important functions in the body‚ such as aiding in digestion and metabolism. (Biology Lab Manual) Some enzymes help
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INTRODUCTION Isolation of Aspergillus niger from garden soil for the production of amylase enzyme and effect of various parameters on enzyme‚ production is the main aim of this study. Enzymes are protein catalysts synthesized by living systems and are important in synthetic as well as degradative process. Amylases are starch degradative enzymes that catalyze the hydrolysis of internal a-1‚ 4-0-glycosidic bonds in polysaccharides such as starch in to simple sugar (glucose and maltose). It is produced
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Concentarion ENZYME LAB Introduction- Enzymes are proteins that speed up the rate of reactions in living things. In this lab‚ we will perform four experiments exploring the way enzymes work. PART A: pH SPECIFICITY Every enzyme has a specific pH at which it works best. In this section‚ you will determine which pH is best for the enzyme‚ catalase. Living tissues produce the enzyme catalase‚ which is able to break down hydrogen peroxide into oxygen gas and water. The reaction is
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Title: Investigate the breakdown of starch at different temperature AIM This experiment has been done to investigate the action of the enzyme amylase on the breakdown of starch. MATERIALS These were the materials used: four starch/ agar plates‚ a marker pen‚ 1mm graph paper ruler‚ 8mm cork borer‚ forceps and template for cutting holes‚ 1% Amylase‚ water‚ incubator set at 5‚ 20‚ 40 and 60 degree Celsius. METHOD This was the experimental procedure carried out: the materials above were collected
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the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature was raised too high the enzyme would denature. In this experiment
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amylolytic enzyme found in saliva released from salivary glands. All the enzymes show optimum enzymatic activity around 37° C which is our body temperature. Any deviation from optimum temperature can influence configuration of enzymes which involves folding of polypeptide for acquiring & maintaining its catalytic & allosteric sites. Decline in temperature modifies the catalytic site & the enzymatic activity is significantly lowered & enzyme is deactivated. However‚ deactivated enzyme can resume its
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The cycle starts when the enzymes strip off the CoA portion from acetyl-CoA and combine two-carbon acetyl group with a four- carbon oxaloacetate to form six-carbon citric acid (hence the other name for the cycle is citric acid cycle). The process of creating citric acid is facilitated by enzyme called citrate synthase. Krebs cycle in its simple form is the process of modifying citric acid in several steps
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Neville Period 1 10/14/12 Enzyme lab Enzymes are biological catalysts that speed up the process of chemical reactions. They are also proteins‚ and most enzymes activities occur within organism. They decrease activation energy‚ energy that is needed to start a chemical reaction. Enzymes are substrate specific substrates ending in "-ase"‚ enzymes ending in "-ase". External factors‚ such as temperature‚ pH‚ and concentration of the substrate‚ affect the enzymes activity in the lab‚ pectase and
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