EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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Porcine Pancreatic Alpha-Amylase Activity Introduction Proteins function in a variety of different ways‚ and one of their fundamental tasks is to act as enzymes. Enzymes are extremely important in controlling reaction speed (by initiating and regulating biological activity)‚ cell communication‚ and growth. One particularly significant enzyme is amylase‚ which catalyzes the hydrolysis of alpha glycosidic linkages of amylose‚ starch components‚ and other oligosaccharides (Qian‚ et al.‚ 1994).
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March 6‚ 2013 Abstract Enzymes are very specific protein because they contain one active site on their surface that enable the substrate to bind to the enzyme and form the enzyme substrate complex and then release the products. The principal function of enzymes is to increase the rate of the chemical reactions. Enzymes have a set of conditions at which they work perfectly; this is known as optimal condition. Fungal and bacterial amylase are the enzymes that we going to study for their
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Fermentation of lignocellulosic hydrolysates involves the conversion of sugars to ethanol which is mainly performed by bacteria or yeast. The organism chosen should possess certain characters in terms of tolerance I‚e towards inhibitors ‚sugars and ethanol concentrations in the hydrolysates and should also withstand higher temperatures and lower pH and with minimal by product formation [161]. Fermentation is the key component where advancement in technology plays key role and is required to be feasible
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Cell Biology- Osmosis‚ Cell Size and Diffusion and Enzymes 1.0 INTRODUCTION Cells are the basic building blocks of all living things. They provide structure for the body‚ take in nutrients from the food‚ convert those nutrients into energy‚ and carry out specialized functions. Cells also contain the body’s genetic material and can make copies of themselves. A cell is also a metabolic compartment where many different chemical reaction occur. There are two types of cells‚ eukaryotic and prokaryotic
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Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity studies
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The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment
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substrate concentration on enzyme function Experiment 2 Exercise 1b – Effect of pH on enzyme function Experiment 2 Exercise 2 – Cellular Respiration and Photosynthesis Experiment 2 Exercise 1A: Effect of substrate concentration on enzyme function Review the Week 2 Experiment Introductions‚ our online lecture on Energetics and pp 80 - 82 in your book. For this exercise‚ we are going to look at the effect of substrate concentration on enzyme function‚ while holding enzyme concentration‚ pH and temperature
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References 31 33 34 35 37 40 43 46 49 I. Introduction How well biocatalysis enables novel routes to target molecules ultimately rests on the properties of the available enzymes. In most applications‚ proteins are called on to catalyze reactions for which they did not specifically evolve. This means one must first identify the best enzyme ‘‘tool’’ for a particular synthetic task‚ and this phase of bioprocess development has often consumed a significant fraction of the total effort expended. Biocatalyst
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