Enzyme Activity Over Different Concentrations and Effects The goals of this experiment were to examine the effectively of enzymes on samples of different enzyme concentrations and substrate concentrations. In addition‚ the experiment tested how effective enzymes are on samples of pH levels and temperature levels. A. Effect of Enzyme Concentration Hypothesis: With half as much enzyme concentration then the reaction rate will be half as much than when the enzyme concentration is equal
Premium Enzyme PH Buffer solution
The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
Premium Enzyme PH Chemical reaction
Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
Premium Chemical reaction Oxygen Enzyme
oD 4.1 Respiration Why organisms undergo respiration What is respiration and why do we need it? Respiration is the process whereby energy is released from food molecules (most usually glucose). It takes place inside living cells‚ both eukaryotic and prokaryotic. The process takes place inside the cytoplasm and the mitochondria. Energy is used to synthesise adenosine triphosphate (ATP)‚ which acts as a short-term energy store in cells. All living organisms need energy to drive their
Premium Adenosine triphosphate Metabolism Cellular respiration
the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that are directly absorbed into the bloodstream during digestion. Materials: 2% yeast solution Large beaker Small beaker Conical flask
Premium Carbon dioxide Glucose Fructose
In the yeast discovery lab we had to decided what the outcome would be then perform the experiment. The experiment was done during class time‚ so everyone’s results would be the same. There were four bottles with warm water in them and to those bottles were added yeast. Then to one of each bottle there was added sugar‚ corn syrup‚ corn starch. To the fourth bottle there was only yeast added and used as a control group. Balloons were then stretched onto the top of the bottles to catch any gas the
Free Carbon dioxide Oxygen Maize
Abstract The effect of nature of substrates on the rate of cellular respiration in yeast was determined by using the Smith fermentation tube method. Mixtures of 15ml distilled H2O‚ 10% yeast suspension and 15ml of the following solutions (all at 10% concentration):1- starch‚ 2 – lactose‚ 3 – sucrose‚ 4 – glucose‚ 5 – fructose‚ 6 – distilled water ‚ were poured in six smith fermentation tubes. Cotton balls were plugged in the openings of the tubes and the tubes were kept upright and observed for
Premium Cellular respiration Carbon dioxide Metabolism
An experiment to demonstrate the effect of temperature on fermentation by yeast The purpose of this laboratory is to observe how temperature affects the metabolism of Grape juice by visibly noting the volume changes of identical food mixes containing yeast at different temperatures. Background Information If yeast is added to a liquid containing sugar and other nutrients‚ kept at an appropriate temperature (and deprived of oxygen)‚ it will turn the sugars into ethanol (alcohol) and carbon
Premium Carbon dioxide Gas Thermodynamics
CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
Premium Enzyme Enzyme inhibitor
Lab 8 Cellular Respiration and Fermentation Objectives: 1. Observe the effects of cellular respiration on temperature in a closed system. 2. Investigate carbon dioxide production in both germinating pea seeds and crickets. 3. Perform an investigative study of the rate of cellular respiration in both pea seeds and crickets at various temperatures. 4. Compare the alcoholic fermentation of glucose‚ sucrose‚ and starch by yeast. Introduction All organisms must have a continual
Premium Carbon dioxide Yeast Adenosine triphosphate