the shape. The next step according to the result‚ will be a catalase test.
Premium Staining Gram staining Bacteria
Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
Premium Hydrogen peroxide Enzyme Oxygen
LAB 1: What temperature does the enzyme actually work properly in? (Hypothesis) If the temperature is below 40 but above 20‚ then the liver will show bubbles. If the temperature is raised higher than the optimum temperature‚ then an extreme decline in enzyme activity would occur following by the quick denaturing of the enzyme‚ rendering it is permanently useless. Also about 37°C is body temperature. The liver that was at 25°C had a huge amount of bubbles (a 4 on the scale) and the 0°C
Premium PH Enzyme Acid
Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
Premium Enzyme Oxygen Catalysis
potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the bottom of the first beaker‚ which contained hydrogen peroxide solution. The timer was then started. The timer stopped when the paper disk floated up to the top of the beaker. This was performed 3 times. After this‚ two negative controls were tested. The first negative control used a denatured catalase instead of the potato extract. The
Premium Hydrogen peroxide Catalase Oxygen
Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
Premium Enzyme Chemical reaction Catalysis
From the graphs‚ it is evident that an increase in both catalase concentration and substrate concentration resulted in a higher rate of reaction or‚ as observed in the kPa graphs‚ a higher volume of O2(g) formed at the end of the 5 minute trial. Interestingly‚ it should also be noted‚ as it was mentioned in the Figure 2‚ that the trend for the 6mL of 3% H2O2(aq) was more of a linear trend than an exponential decay‚ steadily rising until the end of the 5 minute trial. From this‚ it can be inferred
Premium Enzyme Chemical kinetics
Effecting the Reaction Rate in Enzymes in Plants and Animals Problem- How do variables temperature and surface area affect the rate of reaction occurring in the enzyme? Information- Enzymes are proteins that act as biological catalysts. They speed up chemical reactions that take place in cells. Enzymes can be found in both liver and potato. They provide a site where reactants can be brought together to react. These reactants are called substrates that fin into the enzyme. The changing of heat‚
Premium Chemical reaction Reaction rate Liver
Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
Premium Boiling Water Distillation
relationship between catalase concentration and the rate of reaction. This means when catalase concentration increases‚ the rate of reaction also increases. In other words‚ at 20% catalase concentration‚ the rate of reaction was only 4.220 mm/s while at 100% catalase concentration‚ the rate of reaction was 7.704 mm/s. This proves the positive correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to
Premium Oxygen Water Enzyme