Jolly Nguyen 11C Conditions needed for effective enzyme action Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions. Hypothesis: Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases‚ and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins
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Year 11 IB Biology IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will
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what causes starch to thicken or thin which is relevant to obtaining the desired viscosity for recipes that involve starch cookery without complication. Factors to be considered in the thickening power of cornstarch include the concentration of starch‚ extent of gelatinization thus temperature and duration of heat‚ and the addition of sugar or salt (1). Background Gelatinization‚ a physical change‚ occurs when starch is heated in water. Due to the solubility of the amylose in the starch granule
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Describe how the uses of plant fibres and starch may contribute to sustainability How can plant fibres be used? First of all the plant fibres have to be extracted from the plant itself. The process of extraction often used is called retting. Retting involves using water or micro-organisms to dissolve or rot away the cellular tissues surrounding the bast-fibre bundles. Afterwards the fibres are obtained; usually they extract very long sclerenchyma cells and xylem tissue because of their good
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
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Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes‚ temperature‚ and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide‚ then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions‚ meaning more oxygen
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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Enzymes are biological catalysts and each individual enzyme can only catalyse to one type of reaction – due to its specific shape. Each individual enzyme has its own specific shape which is determined by the amino acid sequence that it is made up of – each enzyme’s active site matches to its unique substrate molecule. For the sake of our experiment – enzymes catalyse reactions because they become an active site for reactions to take place. This lowers the energy that is needed for the reaction but
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Enzyme Reaction Rates Under Different Conditions Introduction In this lab you will observe how the rate of a reaction is changed as certain factors are changed. You will observe how changing factors like temperature‚ pH‚ substrate concentration‚ and enzyme concentration changes the rate of an enzymatic reaction. In this experiment you will act as an enzyme by breaking toothpicks witch act as substrates. After observing the results of this experiment you will be able to determine what causes the
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