Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature and also the effect of inhibitors on enzyme activity
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The prokaryotic cell precedes any forms of Bacteria‚ Archaea‚ and eventually Eukaryotes by approximately two billion years. This cell was the original life form on the planet and represents the smallest and least complex of all organisms. Through the process of mutation and adaptation they were able to survive to evolve into our modern day Bacteria‚ Archaea‚ Fungi and Protists. These post organisms have benefited from their early ancestor‚ by way of Endosymbiosis. Intricate protest and fungi are
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to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃. The alternate hypothesis presented
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"Monopoly power is not automatically bad as long as it is regulated". Discuss this view [20marks] Monopoly power occurs when a business is a dominant seller of a good or service with a market share that exceeds 25%. There are many disadvantages for societies where monopolies exist. A higher price than those in competitive markets is one of the main disadvantages for society. As monopolies are the main seller of goods and services in the market they can use their market power in order to raise
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2. Draw and completely label a cell membrane. [pic] 4. List and briefly discuss the 5 components of cellular membranes. 1. Phospholipids layer- 2 layers ‚ polar and non polar ‚ polar facing the extracellular fluid and cytoplasm 2. Transmembrane proteins-integral membrane proteins are proteins that expand the membrane 3. Interior protein network-peripheral proteins are in the interior side of the membrane 4. Cell surface markers are glycolipids‚ carbohydrates/lipid
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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The Cell membrane surrounds all living cells and is the most important organelle‚ there is also a similar plasma membrane that surrounds all the organelles except for the ribosome. The membrane controls how and what substances can move in and out of the cell/organelle The structure of the membrane is often referred to as the “Fluid Mosaic Model”; this is because of the way it is structured It is composed of phospholipids‚ proteins‚ and carbohydrates‚ which are arranged in a fluid
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reaction will be highest. This relate to other resources‚ such as books and the internet‚ which show that the optimum pH value for catalase is pH7‚ therefore there is no difference between the optimum pH and the pH used in the experiment‚ hence the enzyme structure is not affected by the pH and is most active. However the graph inadequately supports the second part of the prediction‚ that the rate will be lowest‚ the more acidic or alkaline the pH level is. For instance when the rate starts of
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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