organism B was oxidase negative. Then I decided I would run the biochemical tests on organism B. I observed that organism B was non-motile and did not produce endospores. With these results‚ I narrowed down the possible species to Enterococcus faecalis‚ Enterococcus faecium‚ Lactococcus lactis‚ Leuconostoc mesenteroides‚ Pediococcus parvulus‚ and Streptococcus mitis. After obtaining some background information on these bacterial strains in the Strain Information handout‚ I decided to inoculate
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blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus faecalis SEM * Erysipelothrix rhusiopathiae on Columbia horse blood agar * Listeria monocytogenes on blood agar * MRSA on Brilliance MRSA Chromogenic Agar (Methicillin-resistantStaphylococcus
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via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse esculin in the presence of bile salts‚ and inhibit growth of other
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of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species.
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Alcaligenes faecalis‚ Rhizobium radiobacter‚ Salmonella typi‚ Serratia marcescnes‚ Shigella flexneri‚ or Yersina pseudo tuberculosis. Ten of the unknown bacteria were classified as Gram-positive bacilli: Bacillus cereus‚ Bacillus circulans‚ Bacillus coagulans‚ Bacillus megaterium‚ Bacillus subtilis‚ Bacillus thuringiensis‚ Brevibacillus brevis‚ Geobacillus stereothermophilus‚ Lysinibacillus sphaericus‚ Clostridium perfringens. Lastly‚ there were also 11 Gram-positive cocci microbes: Enterococcus faecalis
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3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient G Bubbles Positive Micrococcus luteus Bubbles/O2 formed Positive Enterococcus faecalis No bubbles/No O2 Negative Observations: When
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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plates. Streak the plates for the following organisms: Enterobacter aerogenes‚ Salmonella enterica‚ and Staphylococcus aureus‚ and sterilize the loop after streaking each quadrant. 2. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Streak the plate. 3. Repeat step 2 for Streptococcus pyogenes 4. Transfer an A disc with flamed forceps to the cross of first and second quadrant of the
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Makati Medical Center College of Nursing DRUG STUDY Patient’s Name: M.D.C. Age: 42 y/o Medical Diagnosis: Leptospirosis Attending Physician/s: M.T Allergies: none Allergic Responses: none Prepared by: Shiela Guiquing |DRUG NAME |MECHANISM OF ACTION |RATIONALE FOR THIS PATIENT |ADVERSE DRUG EFFECT |NURSING IMPLICATIONS | |
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within the mucous coating on the intestine. They don’t damage their range-land. Dr. Gilmore along with B. Faecalis‚ the research exhibited "that it devotes many of its genetic material to digestive enzymes in which take place on the surface of the cell.” That suggests it can be breaking substances for other additional organism‚ most likely aiding certain sorts of microbial neighbors. Enterococcus might be getting the chance to move nearer to the small intestine or even the urinary tract that is to
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