cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes in the ice bucket A and B. - Add 10 ml of cold isolation buffer and 10 ml of extract to tube A. Mix thoroughly and centrifuge at 3000 RPM for 10 min. (Make sure tubes are balanced before running centrifuge. Weight- balance other tube with water to make it equal to tube A). - Collect supernatant and add to tube B. - Pellet in tube A contains cell debris‚ cells‚ cell wall material‚ nuclei and mitochondria. Add
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Reactions of Three Isomers of Butanol Purpose: To test the reactions of primary‚ secondary‚ and tertiary alcohols with acid and with an oxidizing agent. Equipment and Materials: Chemical safety goggles Lab apron Protective gloves 3 test tubes Test-tube rack Eyedropper Dropper bottles containing the following Butan-1-ol Butan-2-ol 2-methylpropan-2-ol Potassium permanganate solution‚ KMnO4(aq) (0.01 mol/L) Concentrated hydrochloric acid‚ HCl(aq) (12 mol/L) *Alcohols are flammable and
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macroscopic properties of three chemical systems at equilibrium. 2. To observe shifts in equilibrium concentrations as stresses are applied to the systems. 3. To explain observations by applying LeChatelier’s Principle. Materials 12 test tubes test tube rack 2 -100mL beakers beaker tongs safety glasses stand ring clamp wire gauze bunsen burner sparker 2 -250mL beakers 10mL grad cylinder
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Therefore it is vital to use the same beetroot. Volume of water A 25ml measuring cylinder should be used to accurately measure 15ml of water for each test tube. Water is a solvent and only a certain amount of pigment can be absorbed by the water. Hence it would create a contrast in water potential. Time Each cylinder will remain in its test tube for 5 minutes before the water is removed and tested. A stopwatch must be used.
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Hole ’s Human Anatomy & Physiology‚ 9/e David Shier‚ Washtenaw Community College Ricki Lewis‚ The University at Albany Jackie Butler‚ Grayson County Community College ________________________________________ Reproductive System Case Study: Chlamydial Infection Chlamydial Infection Case Presentation Cory‚ a nineteen year old college student‚ has been experiencing some pain for the last couple of days during urination. On one occasion‚ he observed a small amount of yellowish discharge
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water moves from an area of low concentration to an area of high concentration. I am going to record my results to see if osmosis occurs in water solution of sugar solution. Apparatus In this experiment I would need; - 1) 3 test tube 2) A beaker 3) A cork borer 4) A Knife Safety In this experiment safety will not be a major hazard as it’s not a very dangerous experiment apart from the knife which I’m using to cut the potato pieces which I need to be careful
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spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink. Materials: Beakers‚ 50-mL‚ 2-3 ; Test tubes (13 x 100)mm 3-8 ; FD&C Blue 1 stock solution‚ 50-mL ; Lens tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure: 1) Preeminently‚ follow all laboratory safety guidelines ensuring that you have safety goggles on at all times throughout the lab. 2) Fill the
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1. The nurse checks for placement of a nasogastric tube before beginning tube feeding for a client. Which of the following results would indicate to the RN that the tube feeding can begin? The PH of the contents removed from the NG tube is 3 (stomach acid contents are acidic) 2. The nurse in an outpatient clinic teaches a client with right-sided weakness to walk down stairs using a cane. What behavior would indicate by the client that teaching was successful? The client leads with the cane
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was solved with a rectifying vacuum tube‚ invented by John Ambrose Fleming. The vacuum tube was added a new component to become an amplifying vacuum tube. Lee De Forest who was an American‚ invented this amplifying vacuum tube by adding a third electrode‚ called a grid. The grid’s negative potential controlled the flow of electrons from the cathode to the anode. The lower the negative potential of the grid‚ the more electrons it allowed to flow through the tube‚ hence producing an amplified current
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EXPERIMENT 3: IDENTIFICATION OF FOOD CONSTITUENTS IN MILK. INTRODUCTION: In this experiment two milk samples are given and its compositions are tested using different methods such as using benedict solution to test the presence of reducing sugar‚ using copper II sulphate to test the presence of protein‚ using K3( to test the presence of fat. However in this experiment‚ not only are the milk tested for its composition‚ the amount of the particular substance such as reducing sugar‚ protein and
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