Aseptic Technique and Culturing Microbes‚ Lab #3 Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media‚ including MRS broth and nutrient broth‚ learn about oxygen and temperature requirements for microbial growth and how to control microbial growth. Procedure:
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Aseptic Technique and Culturing Microbes Lab 3 Brittney Case August 4th 2013 Section: Mic 101 Microbiology Session 1 Abstract: The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures‚ learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Hypothesis: This exercise will allow me to gain knowledge of how to properly use aseptic techniques
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I. INTRODUCTION Independent Study Questions 1.) What is the fundamental difference between eukaryotic and prokaryotic cells? Procaryotic cells do not contain a membrane-bound nucleus or any membrane-bound organelles. Eurkaryotic cells contain both a membrane mound nucleus and membrane-bound organelles (making them far more complex). 2.) List and describe the two domains of prokaryotic organisms. a. Bacteria- Most are a specific shape (cylindrical‚ spiral‚ or spherical). Cell
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Morales‚ Marinel M. Dr. Annie Cu Gallardo BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some
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Class‚ Euascomycetes; Order‚ Onygenales; Family Onygenaceae; and the Genus Coccidioides. Coccidioides immitis are thermally dimorphic fungus that reproduces by arhroconidia‚ swelling of arhroconidia in vivo into spherules (which burst and release endospores.) The fungi is found mainly in warm dry areas with low rain fall‚ high summer temperatures‚ and low altitude. C. immitis is geographically limited to the san Joaquin valley region‚ and co exists with C. Posadasii in the desert southwest of the United
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ANTIMICROBIAL EFFECT OF BEE’s HONEY IN COMPARISON TO ANTIBIOTICS ON ORGANISMS ISOLATED FROM INFECTED BURNS‚ WOUNDS AND HARMONIZED METHODS OF MELISSOPALYNOLOGY. PROJECT GUIDE: Dr. JAGAN MOHAN REDDY STUDENTS: MANASA.D.A (1AY09BT011) SANJUKTA BANERJEE (1AY09BT019) SANDHYA.S (1AY09BT018) DEPARTMENT OF BIOTECHNOLOGY ACHARYA INSTITUTE OF TECHNOLOGY ACHARYA
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cereus‚ a soil bacterium‚ and B. anthracis‚ the cause of anthrax: the three organisms differ mainly in their plasmids. Zakharyan R.A et al. first reported the presence of plasmids in B. thuringiensis and suggested involvement of the plasmids in endospore/crystal formation. They also described the presence of large plasmid in the Cry+ variant of B. thuringiensis Upon sporulation‚ B. thuringiensis forms crystals of proteinaceous insecticidal δ-endotoxins (Cry toxins) which are encoded by cry genes
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Chapter 1. Microbiology – Diversity of Organisms Microorganisms- too small to be seen with the unaided eye “germ”- rapidly growing cell Microbes in our lives Pathogenic- disease causing Decompose organic waste Producers in ecosystem (photosynthesis) Produce industrial chemicals such as ethanol and acetone Produce fermented foods ( vinegar‚ cheese‚ bread) Produce products used in manufacturing (cellulose) and treatment (insulin) Designer Jeans: Made by Microbes? Stone washing- Tricoderma
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(Saccharomyces)‚ sporangiospores of Rhizopus‚ conidiospores of Penicillium Staining Labs (3-6‚ 3-7‚ 3-8‚ and 3-9) – Identify and correctly interpret the following: Lab 3-6: Gram stain Lab 3-7: Acid Fast stain Lab 3-8: Capsule stain Lab 3-9: Endospore stain Metabolic Tests: Lab 5-1: O-F Glucose (aerobic versus fermentative organisms) Lab 5-2: Phenol Red broth for sugar fermentation (acid and/or gas) Lab 5-3: MR-VP: Methyl red and Voges Proskauer tests Lab 5-4: Catalase Lab 5-5: Oxidase
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Ch. 1 Prokaryotes = bacteria ‚ archaea Eukaryotes = Fungi‚ protozoa ‚ algae (Growth:: Mitosis有絲分裂) Bacterial cell → Bacillus(rodlike) ‚ cocus (spherical)‚ spiral (curved) *P.S. 有時會star-shaped or square → 出現既形式: pairs‚ chains‚ clusters cell walls of bacteria → peptidoglycan (carbohydrate & protein complex) cell walls of plant and algal cell → cellulose Growth::Binary fission = bacteria reproduce by dividing into two equal cells bacteria 吸收nutrients from dead / living organisms/ photosynthesis
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