Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
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1. Microbiology ⁃ M microscope ⁃ I independent unit ⁃ C comparatively less complex ⁃ R rapid rate of reproduction ⁃ O omnipresent ⁃ humans are living repositories of bacteria/microbes ⁃ borne sterile ⁃ microbe on all surface area of the body ⁃ sterile areas: eyes‚ brain‚ spinal cord‚ bones‚ kidney‚ internal organs ⁃ mutualistic relationship: we provide site and nutrient and microbes provide vitamin‚ aid in food digestion ⁃ division of microbial world ⁃ living component: organism
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packing everything it needs to survive until conditions improve. C. tetani is found mostly in warm‚ damp areas‚ especially in soil‚ but can also be found in the intestines or feces of many animals (Wells‚ 1983). The medical importance of bacterial endospores is significant. While it is typically pretty easy to kill off vegetative cells‚ no matter how scary a pathogen they may be‚ bacterial
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Escherichia ● Two physician who notice that infections can be transferred to another human. Holmes and shmmelys ● A sterile environment is free of actively growing cells and microorganisms‚ but may contain viruses or domain life forms such as endospores. False ● A organelle is any small membranebound component found inside of an eukaryotic cell. ● Viruses are living organisms and are classified under the woese system as members of the domain Archaea. False ● A microbial pathogen is co
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and objects that will not melt. Incineration burns organisms and physically destroys them. This method is used for needles‚ inoculating wires‚ glassware‚ etc. Boiling at 100oC for 30 minutes kills almost all endospores. Very long or intermittent boiling is required to kill endospores and sterilize a solution. Toxic chemicals and gas such as formaldehyde‚ glutaraldehyde‚ and ethylene oxide can kill allforms of life in a specialized gas chamber. C. What is pure culture? Why is it important to
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` Study Guide for Exam 1 Chapter 1: 1. What is a “microbe”? What groups of organisms are included in this category? Microbes (microorganisms) are minute living things that individually are too small to be seen with the unaided eye the group includes bacteria‚ fungi‚ protozoa‚ and microscopic algae‚ viruses Only minority of microorganisms are pathogenic(disease-producing) 2. What are some of the benefits provided by microbes? Decompose organic waste‚ producers in the ecosystem
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tetracycline‚ erythromycin‚ and ciprofloxacin. As far as methods of prevention‚ there is a vaccine available for those whom are at high risk‚ for example‚ veterinarians‚ lab technicians‚ etc. One interesting fact about Bacillus subtilis is its endospores can survive up to 6 years in space if coated by dust particles protecting it from solar UV rays
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present
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and purple and green phototrophic bacteria is? a) energy source b) cell wall type c) Oxygen production d) cell type e) color 17) You have isolated a gram-positive rod. What test will you perform next? a) gram stain b) lactose fermentation c) endospore stain d) flagella stain e) DNA fingerprint Short answers. Fill in the blanks or give a short answer for the following. 1) Your field of view through a microscope is 0.3 millimeters. If you have a worm that takes up half of your field of
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