Bacterial cells are Prokaryotic cells. These cells have several internal structures and surface structures. The cell walls are of two types‚ gram positive and gram negative. Out of all the structures‚ the two selected structures are the cell- wall and endospores. The cell- wall of bacterial cells is found in three distinct shapes such as spherical‚ spiral and rod shaped. The cell wall of the bacterial cells is composed of the proteins and polysaccharides. This is collectively called peptidoglycan. The cell-
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University of Phoenix Material Name:Shaniece Daniels Appendix A – Cell Division in Bacteria After reading Ch. 6 in Microbiology: Principles and Explorations‚ fill in the following matrix. List the four phases of bacterial growth and briefly describe what happens in each phase. Lag phase: Is the period when the bacteria are adjusting to the environment. Log phase: Is when the population grows in a logarithmic fashion. Stationary phase: a substance that shows different
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Control of Microbial Growth: definitions Sterilization: Killing or removing all forms of microbial life (including endospores) in a material or an object. Heating is the most commonly used method of sterilization. Commercial Sterilization: Heat treatment that kills endospores of Clostridium botulinum the causative agent of botulism‚ in canned food. Does not kill endospores of thermophiles‚ which are not pathogens and may grow at temperatures above 45oC. Control of Microbial Growth: definitions
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1320 HBL for CP2078 (Sample Preparation for Microscopy) Name of students Admission No Class Total Marks / 45 Assignment 1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink
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Chapter 1 - Which one of the following is not a role for bioremediation? Curing infectious diseases - All of the following are examples of new emerging infectious diseases except Chickenpox - Normal microbiota: Beneficial microbial inhabitants of the body - Golden Age of Microbiology: Rapid discovery of basic microbiology principles - Re-emerging diseases: Once controlled by preventative public health measures they are now on the rise - Prions: Resistant to the usual sterilization procedures
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NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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bacterium were taken after 48 hours of incubation at 35° Celsius. Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in
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would Gram negative bacteria be after the alcohol wash before the counter-stain? In performing the spore stain‚ why is it necessary to heat the malachite green? When performing the Endospore stain‚ what color do the bacteria appear at the end of the stain? Are the bacteria the same color as the endospore‚ briefly explains? Briefly define aseptic technique. Why is it necessary to heat a loop to redness when flamed? Why is it necessary to cool a loop after it has been flamed BEFORE
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that
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and food products may become contaminated‚ leading to the transient colonization of the human intestine. Illness associated with B. cereus can occur when heat-resistant B. cereus endospores survive cooking. If the food is then inadequately refrigerated or held for extended periods at improper temperatures the endospores can germinate and multiply. Once the spores germinate‚ the vegetative cells can multiply and produce illness causing enterotoxins. Bacillus cereus is known to cause two distinctly
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