effective for stopping tumour vascularisation. In this study‚ the pericarp of the Calabash (Crescentia Cujete) is utilized as an effective treatment for stopping tumour vascularisation.The obtained 457g of Calabash Fruit pericarp was soaked by solvent extraction using 1.5L 95%-Hexane and 1.5L 95%-Ethanol for 24 hours each. The extract was filtered and evaporated using rotary evaporator for 3 hours and further dried for 24 hours. The residual crude extract was poured in a glass container. The extracts were
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Biological Molecules: Carbohydrates‚ Lipids‚ Proteins By the end of this lab‚ the student should be able to: Identify the functional groups for each of the biomolecules that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Brown Bag test‚ Sudan III/IV test‚ and the Biuret’s Test. Describe the mechanism of reaction for: Benedict’s test‚ Iodine test‚ Sudan III/IV test‚ and the Biuret’s Test. Interpret the results when presented with data for each of the biochemical
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donor compensation‚ it is estimated that the research can cost $1000 per egg (Mombaerts P‚ Forbes). Furthermore‚ depending on the disease being researched‚ up to 1.5 billion eggs could be required (brighthub). That would necessitate a minimum of 500 000 willing volunteers for that disease alone (brighthub). Additionally‚ donors can experience significant adverse medical and psychological risks associated with the egg extraction process. For example‚ women risk contracting serious illnesses such as
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ACTIVITY: THE CAT’S MEOW / MILK CO-MOTION PURPOSE: To observe the interaction of detergent with the fat of the milk and food color 1. Provide a possible explanation of phenomenon you observed based on the result of your experiment. When food color is added to milk‚ it floats because it is less dense than milk. The non-polar fat in the milk prevents the food color from spreading‚ as it cannot participate in hydrogen bonding with water. When detergent is added to milk and the food color‚ the
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CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8.8 and stacking gel‚ prepared using
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EXPERIMENT NO. 3 DENATURATION OF PROTEINS Abstract The experiment aimed to use the concept of viscosity to study the effects of different denaturants on 1% albumin extract. An Ostwald viscometer was used to measure the flow time of 5 mL of the blank and native protein. These were then denatured by adding 1 mL of denaturant and had their flow time measured. The flow time from the blank to denatured protein is
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Even though we were successful in purifying LDH enzyme‚ further steps can be taken to increase the yield. Materials and Methods Cell Lysis and Extraction of LDH: Approximately 40 g of minced chicken breast meat (40.327 g) is blended with 75ml cold extraction buffer in four 30-seconds bursts for homogenation of the muscle tissue. The extraction buffer contained 10mM Tris-HCl (pH-7.4)‚ 1mM 2-Mercaptoethanol‚ 1mM Phenylmethylsulfonylflouride (PMSF)‚ 1mM Ethylene diamine tetraacetic acid (EDTA)
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diglycerides‚ triglycerides‚ phospholipids‚ and others. In this experiment lipid was extracted from the egg yolk of a chicken egg. Three forms of lipid were extracted namely total lipid‚ neutral lipid and polar lipid. Then using a square chromatoplate the lipid was subjected to a two-dimensional thin layer chromatography to separate the lipid into different components. Theoretically the egg yolk should contain TAGs‚ cholesterol ester‚ cholesterol‚ free fatty acids and polar lipids. In the end of
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place them in the front of the room for easy access for students: a. Polysaccharide Solution - blended potato or lab grade starch solution b. Monosaccharide Solution – apple juice or lab grade glucose solution c. Protein Solution – blended meat or egg whites d. Lipid Solution – vegetable oil‚ melted butter 2. Set up 4 lab stations (twice around the room) for students to rotate. Each station should have the materials needed to conduct one of the following tests: a. Iodine Test: starch + iodine (yellowish
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Paclitaxel (PTX) Paclitaxel (PTX)‚ a member of taxane family‚ it’s an effective antineoplastic agent for treatment of many forms cancer. The success of PTX in these diseases due to a broad spectrum of antitumor activity‚ efficacy in terms of dense disseminated tumours and a unique mechanism of action. This microtubule-stabilizing drug selectively disrupts the microtubule forces‚ which induces mitotic arrest that leads to cell death(Bernabeu et al. 2017). A brief run through of its development from
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