Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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The Effects of Heat on the pH of the Vegetable and Their Pigment PURPOSE The purpose of this experiment is to see how pH levels of certain vegetables change while being cooked in four different mediums (frying‚ boiling‚ steaming‚ roasting)‚ and how the varying pH levels change the coloring of the vegetables. We will analyze how the different method of cooking/heating changes the levels of pH. BACKGROUND INFORMATION This experiment is based around the different pigments in vegetables‚ of which
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The pH Scale is a scale made to show how acidic or alkaline a substance is. The pH of something is measured with pH paper which can vary from wide range paper‚ which covers all ph’s‚ but is not as accurate at some others. There are pH papers that get very specific that can have a range such as 2 or 3 and get much more precise. The neutral on the scale is 7. Anything that is below 7 is considered acidic and anything above 7 is considered alkaline. Acidic substances contain more H+ than OH-. H+
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AbstractDifferent pH level may affect the growth and development of the plants. Certain key words include: pH level‚ germination‚ acidity‚ osmosis and diffusion. This experiment examines the effects of different pH level of vinegar on the growth of bean plants. Materials used in this experiment consist of: water (pH 6)‚ vinegar with the pH level of 3‚ 4 and 5 (each one were made before experiment)‚ beans‚ soil‚ and pots. Eight bean plants were planted‚ two were watered with pH 6 and the other ones
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The Effect of pH on the Rate of Osmosis Using a Glucose Solution Melissa Werderitch Biology 157 11/6/06 Introduction In a journal article written by Florian Lang‚ osmosis is essentially explained as the flow of water from one area to another that are separated by a selectively permeable membrane to equalize concentrations of particles in the two locations (Lang‚ 1997). Osmosis is able to maintain osmotic pressure and regulate a cell’s volume. In a hypotonic () or hypertonic () environment
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1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab‚ and conduct the experiments provided before going out into the virtual field for additional research. Please type your answers on this form. When your lab report is complete‚ submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section 1: You will be testing 4 known solutions for pH levels using a standard wide-range
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A pH meter is an electronic instrument used to measure the pH (acidity or alkalinity) of a liquid (though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading. The probe The pH probe measures pH as the activity of hydrogen ions surrounding a thin-walled glass bulb at its tip. The probe produces a small voltage (about 0
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Effects of Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical
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In this lab‚ I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than
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