Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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PH at the UN Alfredo Vidal Ceballos 7/10/2014 Group Support Part 1: Solution Group Name and M. cons. Chem formula Type Experimental pH 1 Deidre Acetic Acid 0.1M HC2H3O2 Weak Acid 3.03 3.03 2 Acetic Acid 1M HC2H3O2 Weak Acid 2.59 2.54 3 Hydrochloric Acid 0.5M HClStrong Base 0.85 0.90 4 Jonah Nitric Acid 0.1M HNO3 Strong Acid 2.14 2.14 5 Sodium Acetate 1M NaC2H3O2 Weak Base 9.42 9.42 6 Potassium Dihydrogen Phosphate 0.1M KH2PO4 Weak Acid 5.47 5.47 7 Andy Potassium Hydrogen Phosphate 0
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by a cold catalase solution and the other in which the Hydrogen peroxide(H2O2) was denatured by a hot catalase solution. The experiment conducted displayed signs of experimental error as there were many situations in which the materials provided could have faulted. Theoretically speaking‚ from the inconsistent values provided for the pH levels‚ a possible error could be that the pH probe may have been internally damaged such that the filling solution was contaminated. Moreover‚ the pH probe although
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
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pH pH means Power of Hydrogen or Potential of Hydrogen. pH is a scale of acidity from 0 to 14. It tells how acidic or alkaline a substance is. More acidic solutions have lower pH. More alkaline solutions have higher pH. Substances which are not acidic or alkaline i.e neutral usually have a pH of around 7. Acids have a pH less than 7. Alkalis have a pH greater than 7. There are a lot of ways for finding the pH of something. One way is to use litmus paper. The pH paper is able to tell how strong
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Arnesh Kaewloyma 9/30/14 Enzyme Catalase under optimum conditions‚ speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of
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launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach)‚ Brassica oleracea (Broccoli)‚ Solanum tuberosum (Russet Potato)‚ Malus domestica (Apple)‚ and Allium cepa (Onion). The five different catalases from the sources will all be used
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of solutions and test their pH. Due to materials available pH strips were the only testing tools available. A table is setup to compare the strip color to its pH level. Four known solutions including bleach‚ lemon juice‚ soda‚ and baking soda were used. There was also an unknown solution in which the pH level was used to determine which solution it was. The pH scale is 1-14‚ a pH of 7 is neutral‚ less than 7 is acidic‚ and greater than 7 is acidic. Materials: * pH strips * 20 test tubes
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pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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