Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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Amino acids are important building blocks; they play an essential role in the formation of proteins and as intermediates in metabolism. Amino acids can serve as cell signaling molecules and can regulate gene expression. An optimal amount of amino acids is essential for health. There are over 500 amino acids‚ however only 20 are commonly found. Amino acids all have an amine functional group and a carboxylic acid functional group. They differentiate from each other in their side chains. Only 10 of
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Introduction: The theory behind the extraction of a solution containing benzoic acid‚ cellulose‚ and methyl orange involves many components pertaining to the fundamental ideas of solubility and polarity. Using the concepts of like dissolving like and acid base reactions‚ a solution of organic acid (benzoic acid)‚ a water soluble compound cellulose‚ and an organic soluble compound methyl orange‚ can be separated and benzoic acid can be isolated by a method of extraction. At the fundamental level‚
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Purpose: To find out if the Unknown substances are bases or acids. Materials: goggles aprons paper for data table graduated cylinder unknown I substance beakers/test tubes stirring rod litmus paper pH indicator paper pH color chart phenolphthalein scoopula eye-dropper digital pH meter magnesium ribbon calculator unknown II substance forceps sodium bicarbonate Procedures: Test the pH of the Unknown substances with litmus paper and pH indicator paper and match to color chart
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fermented from sugar. The fermentation of ethanol results in the production of acetic acid (CH3COOH). The typical pH of vinegar ranges anywhere from 2 to 3.5‚ although shop-bought vinegar usually measures 2.4 www.wisegeek.com/what-is-vinegar.htm fig 1 In this study we will determine the amount of acid in a vinegar sample by using titration‚ a common technique in chemistry a typical shop brought vinegar has an acid percentage of 5% see fig 1. A titration is a technique where a solution of known
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of Shikimic Acid‚ intermediate for the production of Oseltamivir‚ the Avian Flu drug Supervised by: Dr. Reza Yegani Dr. Mohammad Hossein Sarrafzadeh Mr. Habibollah Ramezanzade by: Kaveh Yazdifard Presented on: 1385/10/3 Contents Acknowledgement Introduction Shikimic Acid & its Natural Properties Derivatives of Shikimic Acid Avian Flu Sickness‚ Medications Oseltamivir and Shikimic Acid’s Role in Synthesis Production of Shikimic Acid Extracting Shikimic Acid from the Plant
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Abstract The objective of this experiment was to observe multi-step purification of benzoic acid after performing the extraction from a mixture containing benzoic acid‚ cellulose‚ and methyl orange. Recrystallization was done to remove impurities from the sample. The amount of BA recovered during recrystallization is much less than the extracted amount of BA. The difference between the pure and impure samples was observed by comparison of melting points. It was found that impure samples will have
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The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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TEABAG EXTRACT AS AN ALTERNATIVE FOR INK An Investigatory Project Chapters 1- 3 Submitted by: Alfonso‚ Manuela Fairbanks Garcia‚ Tonica Paga Cho‚ Jung Hun Lat‚ Manuel Miguel Feliciano November 2013 Chapter I Introduction A. Background of the Study The group will conduct the study because the group noticed that teabag extract is dark in colour and it can be use to make ink‚ and also the group noticed that teabags are made from natural resources. Some kinds
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