You have learnt many staining techniques through the lab exercises: What dye did you use in simple stain? Crystal Violet What dyes did you used in Gram stain? Crystal violet‚ gram’s iodine‚ and safranin violet. Why did you have to heat up the slide when you did the acid fast stain and the spore stain? To open the spores and allow the dye to enter and stain. Once cooled down the spores closed trapping the dye inside. What color is a “+” in acid fast stain? Which bacteria are “+” for
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effective antibacterial agent‚ belongs to the class of azo dyes‚ which were the first commercially produced chemicals; they effectively dyed wool fibers (animal protein). Because they contain N functions‚ the dyes were screened in vitro for antibacterial activity; of over 10‚000 tested dyes none showed any antibiotic activity. However‚ in vivo studies with mice‚ that had been infected with a bacterial culture‚ showed that several dyes counteracted gram-positive bacterial infections. HDR-F-2013
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observations of the reactions were recorded in Data Table 1. A1) Two drops of NaHCO3 and two drops of HCl. B1) Two drops of HCl and two drops of BTB. C1) Two drops of NH4OH and one drop of BTB. D1) Two drops of HCl and two drops of blue dye. A2) Two drops of blue dye and two drops of NaOCl. One drop of HCl was then added. F) Two drops of KI and two drops of Pb(NO3)2. G) Two drops of NaOH and two drops of phenolphthalein. H) Two drops of HCI and two drops of phenolphthalein. B2) Two drops of NaOH
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buffer provided the correct pH for the gel to run. The solution would be heat until the agarose is completely dissolved. Ethidium bromide solution would be added to the solution after it cooled slightly. Ethidium bromide solution was a fluorescent dye that intercalates between bases of nucleic acids and allows very convenient detection of DNA fragments. It enabled visualization of the fragments within the gel under UV light. The solution would be poured into the gel mold for solidification. The gel
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Warmth (comfort) Absorbency (comfort) Comfort (comfort) Handle and drape (aesthetics) Strength (functional) Elasticity (functional) Aftercare (functional) Natural fibres Wool Warm to wear. Slow‚ can absorb its weight in water and not feel wet. Repels water droplets. Very slow drying. Fine wool‚ very comfortable. Coarse wool‚ scratchy. Very soft or coarse handle. Good drape. Medium strength‚ not durable. Very good. Creases drop out. Wash and iron with care‚ may shrink. Dry
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include ultrasound‚ computerized tomography (CT) scan and magnetic resonance imaging (MRI). Using a scope to inject dye into the pancreatic ducts. Endoscopic retrograde cholangiopancreatography (ERCP) uses a dye to highlight the bile ducts in your pancreas. During ERCP‚ an endoscope is passed down your throat‚ through your stomach and into the upper part of your small intestine. A dye is then injected into the pancreatic and bile ducts through a small hollow tube (catheter) that ’s passed through the
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identified. For example‚ at 100x‚ a direct stain of yeast returned a cluster of cocci. A stain is a chemical that adheres to structures of the microorganism and in effect dyes the microorganism so the microorganism can be easily seen under a microscope. Stains used in microbiology are either basic (direct) or acidic (indirect). Basic dyes are used for positive or direct staining
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dish • Incubator • Refrigerator • Bunsen burner • Gas connection • Plastic tubing • Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet dye • Gram’s iodine • Ethyl alcohol • Safranin dye • Paper towels • Wire rack • Sink • Brightfield compound microscope • Lens paper • Immersion oil • Pen and paper Methods I. Collecting the environmental specimens: 1. Place some
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relatively quick and simple process‚ and the results helped to determine its accuracy (Bradford‚ M. 1976). Important aqueous solutions used throughout the experiment were distilled water‚ Coomassie brilliant blue G-250 (CBBG) and BSA. The acidic CBBG dye was used to stabilize the binding of this to the BSA‚ therefore increasing the maximum absorption from 465nm to 595nm (Spector‚ T. 1978). The spectrophotometer is an absorbance measuring instrument that produces light through two bulbs; the first a
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