The objective of these labs were to use sophisticated techniques to produce double-stranded RNA that was incubated with live Drosophila cells to inhibit the expression of our two genes of interest. The overall process of the four labs was to isolate and amplify DNA using the polymerase chain reaction with primers that contained gene-specific sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA
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AS/Foundation geography Coursework Investigating downstream changes on the Afon Caerfanell INTRODUCTION AIM: To investigate how selected parameters change downstream on the Afon Caerfanell. Hypothesis: 1. The velocity of Afon Caerfanell increases downstream 2. The velocity of the river increases down the stream as the angle of the slope increases. RIVER DEFINITION A river is the natural course of
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and max opening scene analysis The Adam Elliot’s claymation Mary and Max opens with credits on a black screen with sad contemplative orchestral music. Then the screen if filled with darkness‚ followed by climbing upbeat hopeful music illuminating a sepia coloured ordinary Australian suburban landscape. Many typical Australian domestic icons portray this: like the Australian rules Sherin branded Football on the roof‚ the thongs protruding from a rubbish bin on Lamington street and a barbeque on a back
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It used to belong to my mother…It’s the sepia color real silk takes on with wear…I’m wearing a leather belt with it‚ perhaps a belt belonging to one of my brothers” (Duras‚ 3‚ 4‚ 11). Duras creates this photographic image of a young “fifteen and a half” (20) dressed in immodest clothing that invites intrigue and suggestion of a feminine body reaching maturity. Sensuality is expressed in the wearing of her mother’s dress. The “transparency” of the “sepia” colored “real silk” dress is extremely sensual
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The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Genetics of Drosophila First‚ Drosophila is the common fruit fly. The common fruit fly is some of the best organisms to study for genetic research and experimentation. This is true for a few reasons‚ the first of which is the small number of chromosomes which is 4‚ and that we have identified the genome. This means all traits are known and we can figure out where mutations take place. The second reason is the fly’s ability to reproduce quickly. A new generation is produced every week‚ allowing
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An Introduction to Peroxisomes: “Peroxisomal Proteins and Pathways in Drosophila” Discovered in the 1950’s‚ peroxisomes were found floating within a cell’s cytoplasm using electronic microscopy by J. Rhodin (Schluter‚ 2010). These were later classified as organelles and the functions of these organelles were also further discovered and understood. Peroxisomes are membrane-bound organelle that are found in all eukaryotic cells participate in many metabolic reactions within the cell. These reactions
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Bibliography: Wikipedia‚ (2005). Drosophila melanogaster. [Online] Available: http://www.answers.com/topic/drosophila-melanogaster-1 [August 20‚ 2005] Gregory‚ E. (Ed)‚ (2000). Nelson Biology VCE Units 3 and 4. Nelson: Melbourne. Manning‚ G. (2005). Introduction to Drosophila. [Online] Available: http://ceolas.org/VL/fly/intro.html [August 20‚ 2005] Manning‚ G. (2005). About Drosophila. [Online] Available: http://www.ceolas.org/fly/ [August 20‚ 2005] Unknown
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BIOLOGY 207 SIMULATOR ASSIGNMENT MULLIS POPULATION WRITTEN RESULTS SECTION In vial one‚ a wild type hyper female Drosophila and wild type mellow male Drosophila were crossed respectively to determine the dominant behavioural phenotype. The first reciprocal cross was then carried out in vial two to define whether or not the behavioural phenotype is x-linked recessive. According to the results obtained from vial one‚ when the wild type hyper female and wild type mellow male was crossed‚ the resulting
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The purpose of this experiment was to determine the F1 genotype of fruit fly traits using the phenotypic ratio of the F2 generation and to express these results of the unknown cross through a Chi-square model and Mendelian genetics. After the experiment‚ it was found that the parents held a heterozygous genotype through using the Chi-square model‚ and that the observed and expected values fall within the Chi-squared value which also falls into the p-value. The Chi-squared value was 5.64‚ the degrees
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