scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards
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QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended in the lysis solution. They were rinsed in a buffer before going through centrifugation. - Polymerase Chain Reaction (PCR): The isolation done‚ the Drosophila cDNA located in the plasmid DNA was amplified. The PCR was used to determine the size of the cDNA inserted into the plasmid DNA. One tube (A) contained the plasmid DNA A with the PCR master
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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William Perez Cell Biology 2440 Lab on protein Myosin Proteins are chains of amino acids that perform the most important functions in living organism. Every protein will contain an amino group‚ carboxyl group‚ a different R group and an alpha carbon with two hydrogens. There are nine types of functions proteins can have‚ enzymes‚ motor‚ receptor‚ structural‚ storage‚ transport‚ signaling‚ and special purpose proteins(antibodies). There are four levels of protein structure‚ primary‚ secondary
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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In this experiment‚ we analyzed the regeneration of planaria over a two week period. We chose to use flatworms due to their ability to grow into a new animal if cut in half or several pieces. This reproductive ability to regenerate is called fission and occurs in flatworms due to their embryonic stem cells called neoblasts. We obtained the flatworms used in this experiment by first placing a few drops of spring water on a petri dish so that the flatworms will be able to survive a couple of weeks
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Biomes‚ every single spices lives in one. Biomes are large naturally occurring community of flora and fauna occupying a major habitat. They can be found over range of continents and have common characteristics with plants and animals. There are many types of Biomes on earth. BIomes are distinct from habitats‚ because any biome can comprise a variety of habitats. In our lab we had figure out the major components in a biome. For example‚ like the weather‚ what animals live in it‚ plant life‚ etc… And
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Introduction: There are times when it is necessary to go through something both inside and outside‚ literally‚ to learn. This was exactly the case with virtual lab #11 where the experiment called for an examination of an earthworm’s exterior anatomy and interior anatomy. The earthworm as a whole is considered the dependent variable as we cannot change it. However‚ the labels to be placed can be moved so they are the independent variables. My hypothesis is quite elementary. I believe that after studying
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4. Results No hypotheses in the discussion section were necessarily falsified‚ but some broad statements were made. For example; “Culture conditions obviously influence the outcome of adaptive evolution.” This statement implies that they have extensive knowledge on all the different aspects of evolution. Many scientists today will admit that we do not fully understand evolution and all the factors involved. To say that the conditions ‘obviously’ influenced that outcome is too broad of a statement
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Cost Audit Report and Compliance Report in XBRL format and what is the authority for the same? Ministry of Corporate Affairs has mandated filing of the Cost Audit Report and Compliance Report from the financial year 2011-12 onwards (including overdue reports relating to any previous year) by all cost auditors and companies concerned by using the XBRL taxonomy. MCA vide Circular No. 18/2012 dated July 26‚ 2012‚ has extended the last date of filing of cost audit reports and compliance reports with the
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