1967 - Enzyme DNA ligase isolated. 1969 - Shapiero and Beckwith isolate the first gene. 1970 - First restriction enzyme isolated. 1972 - Paul Berg creates the first recombinant DNA molecules. 1973 - Cohen and Boyer create first recombinant DNA organisms. 1977 - Karl Illmensee claims to have created mice with only one parent. 1979 - Karl Illmensee makes claim to have cloned three mice. 1983 - Kary B. Mullis develops the polymerase chain reaction technique for rapid DNA synthesis. 1983
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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Main topics on midterm labs 1-9 lectures 1-9 Operon; bacterial promoter; Rbs; DNA polymerase vs RNA polymerase function (lec #1) - Transcription is carried by RNA polymerase that RNA polymerase recognizes to a specific sequence (the promoter) and start making mRNA next to that position. - Usually‚ typical bacterial promoter carries tow consensus sequences (the sequence that all the organism shares) TTGACA at position of -35. TATAAT at -10. - As mRNA is being transcribed
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nucleotides) 4. In prokaryotes‚ DNA molecules are located in the c. cytoplasm (no nucleus) 5. The diagram below shows the process of DNA a. Replication 6. The main enzyme involved in linking individual nucleotides into DNA molecules is d. DNA polymerase 7. The process by which the genetic code of DNA is copied into a strand of RNA is called b. transcription 8. In messenger RNA‚ each codon specifies a particular c. amino acid 9. Changes in the DNA sequence that affect genetic information
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Figure 20.1 1) Which enzyme was used to produce the molecule in Figure 20.1? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase Answer: C Topic: Concept 20.1 Skill: Application/Analysis 2) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with
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Recombinant DNA Technology How to Mess with DNA for Fun and Profit OR OR How to Make a Mouse Glow in the Dark What the heck is Recombinant DNA? Recombinant DNA is what you get when you combine DNA from two different sources. For example: Mouse + Human DNA Human + Bacterial DNA Viral + Bacterial DNA Human + (other) Human DNA (It’s sort of like Frankenstein-DNA!) Why Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify
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molecular biology technique that is used to make specific‚ intentional and precise changes to the DNA sequence of a gene or any gene products. It is also used to determine the structure and biological activity of DNA‚ RNA and protein molecules. It is also called as site-specific mutagenesis or oligonucleotide-directed mutagenesis. This method has also found use in understanding important genetic process like DNA conformational analysis and also functioning of processes like transposition and site-specific
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Epigenetics: interaction of DNA methylation and chromatin Epigenetics is a field where advances are being made daily. Epigenetics is defined as “heritable changes in gene expression that occur without a change in DNA sequence‚” as stated by Dr. Alan Wolffe. A way in which we can understand this definition is by taking the analogy of a card game. The cards‚ the DNA sequence‚ have been dealt and will not change‚ however we need to understand how to play the cards‚ the rules‚ which is epigenetics
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Discuss the chain of events that led to the discovery of DNA as the heritable substance and the discovery of the structure of DNA. Ans> The chain of events led to the discovery of DNA as the heritable substance and the discovery of the structure of DNA. In the late nineteenth century‚ a German biochemist Friedrich Miescher found the nucleic acids which are long-chain polymers of nucleotides‚ and are made up of sugar‚ phosphoric acid‚ and several nitrogen-containing bases. Later it was found
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Module 1 |Long answers 1 | |Question 1 | |Question 2 | |Question 4
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