structure of DNA‚ its nucleotide sequence and how genes are read and regulated. One key tool is the ability to visualize DNA molecules and determine their length by using a technique called gel electrophoresis. Introduction to gel electrophoresis In gel electrophoresis‚ DNA fragments move through a porous matrix made of agarose‚ a gelatin-like substance purified from seaweed. The agarose is melted like Jell-O and then ® poured into a plastic tray to harden into a slab called a gel. A plastic
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DNA fingerprinting is a method that compares the fragments of DNA. DNA fingerprinting was first invented to detect the presence of genetic diseases. Today‚ DNA fingerprinting is used in different ways. DNA is analyzed using a Southern Blot‚ which allows scientists to observe the base pair patterns. DNA fingerprinting can be used in a few different ways. First‚ to find out if the child belongs to a person DNA fingerprinting may be used. When a child is born‚ it inherits the VNTR’s from the father
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MOLECULAR LABORATORY REPORT BIO 615 Name: NUR LISMA RUHILA BT ALIAS Group: AS201 5A Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER
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OUTLINE What is PCR and Gel Electrophoresis? • Polymerase chain reaction (PCR) is a technique which is used to amplify the number of copies of a specific region of DNA‚ in order to produce enough DNA to be adequately tested. This technique can be used to identify with a very high-probability‚ disease-causing viruses and/or bacteria‚ a deceased person‚ or a criminal suspect. • Gel electrophoresis is a widely used technique for separating electrically charged molecules. It is a central technique
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INFORMATION Storage: Upon receipt of the kit‚ store HaeIII restriction enzyme‚ PTC primer/loading dye mix‚ and DNA marker pBR322/BstNI in a freezer (approximately –20°C). All other materials may be stored at room temperature (approximately 25°C). Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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Restriction Enzymes and Electrophoresis Bhumik Patel Phillips 1/16/11 Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough‚ while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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anthropologists ran a gel electrophoresis with DNA from Skeleton 3 and two missing persons‚ Julia Ly and Teresa Chen to help in DNA identification. This process would allow restriction enzymes to cut by a specific restriction site and run through the gel‚ where the DNA fragments would move from the negative side to the positive side of the gel due to the negative charge of the phosphate group in DNA. The smaller the DNA fragments‚ the further they move down the gel. As mentioned above‚ the DNA that was collected
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Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
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