Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA from another source
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The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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February 2013 Gel Electrophoresis Introduction: Agarose Gel Electrophoresis is a process in which the process of determining whether a strand of DNA is either positively or negatively charged. The container in which the gel is stored has a negative and positive side; whichever side the DNA molecules go to means the DNA is charged the opposite way. (Ware‚ Lunte‚ Gardiner)For example if a DNA molecule goes to the negative side that means that the DNA is positively charged and vice
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Should all people convicted of a crime have their DNA fingerprints stored on a database?\ A DNA fingerprint is the same for every cell‚ organ and tissue in an organism. DNA fingerprinting has many uses‚ some of which include providing the evidence needed to solve criminal investigations‚ determining genetic relationships and solving paternity disputes. DNA fingerprinting has many benefits in the use of criminal investigations as it can provide the evidence to solve crimes and current mysteries
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In gel electrophoresis‚ DNA fragments move through a porous matrix made of agarose‚ a gelatin-like substance purified from seaweed. The agarose is melted like Jell-O® and then poured into a plastic tray to harden into a slab called a gel. A plastic comb inserted at one end while the gel is hardening forms wells where DNA samples can be placed. The DNA is mixed with a loading buffer that contains glycerol—this makes it heavier than water‚ so it will sink to the bottom of the well. The gel is then
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DNA FINGERPRINTING DNA fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid. It is a technique used to distinguish between individuals of the same species by using only samples of their DNA. It is also called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. In mammals‚ the strands of DNA are grouped into structures called chromosomes. Unless dealing with identical twins‚ the complete DNA of each individual is unique
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