Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA is a polymer‚ which is a chemical compound or a mixture of compounds consisting of repeating structural units. These repeating structures are created through polymerization. The monomer‚ meaning one part‚ units of DNA are nucleotides. Each nucleotide consists of a five carbon sugar‚ also known as deoxyribose‚ and nitrogen containing base attached to the sugar
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life (1). Significant developments have been made in gene monitoring techniques specifically in DNA microarrays which only very recently revolutionized genome expression analysis (1). Despite continuous improvements and modification to the technique‚ DNA microarrays are still no more than a glass microscope slide studded with individual immobile nucleotide fragments (1‚ 2). The fundamentals of DNA microarrays are set on complementary base-pairing (3)‚ and because the exact sequence and position
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DNA Sequencing As of last few weeks‚ the transformation lab is performed to convey and purify a given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two
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DNA Cloning ~ The Future of Science DNA Cloning DNA cloning is the process where an exact replica of an organism is produced. Some scientists have already produced clones of animals; Dolly the sheep is one of the many examples of DNA cloning. Cloning is a natural process‚ many living things only need one parent to reproduce‚ this process is called asexual reproduction; for example single-celled bacterium use this process. The new bacteria inherits its genes from only one parent‚ this means
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DNA Barcoding Click on the following link and view the background on DNA barcoding: https://www.dnalc.org/resources/animations/dna-barcoding.html 1. What is a DNA barcode? DNA barcoding is a fast accurate method of identifying plants and animals‚ or products made from them. DNA barcode is DNA sequence that uniquely identifies each species of living things. Short DNA sequences are used to identify species by comparing them with the known barcodes in large databases. When you get to the section where
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and the discovery of DNA Ralf Dahm* Max Planck Institute for Developmental Biology‚ Department 3 – Genetics‚ Spemannstr. 35/III‚ D-72076 Tubingen‚ Germany ¨ Received for publication 5 October 2004‚ revised 17 November 2004‚ accepted 20 November 2004 Available online 21 December 2004 Abstract Over the past 60 years‚ DNA has risen from being an obscure molecule with presumed accessory or structural functions inside the nucleus to the icon of modern bioscience. The story of DNA often seems to begin
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DNA and the Gene: Synthesis and Repair 1) Watson and Crick elucidated the structure of DNA in 1953. Their research built on and helped explain the findings of other scientists‚ including ________. A) X-ray diffraction studies by Rosalind Franklin and Maurice Wilkins. B) Chargaff’s rules: C = G and T = A. C) Scientists who recognized that a nucleotide consisted of a sugar‚ a phosphate‚ and a nitrogen-containing base. D) All of the above were important considerations in the elucidation of
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DNA and Evidence DNA is one of the most important roles to evidence and in a criminal case. It helps to prove a convict guilty or help those wrongly accused or convicted. DNA stands for deoxyribonucleic acid. Just about every cell contains DNA. The DNA that’s in people blood is also the same DNA in people’s hair‚ bone‚ saliva‚ skin‚ tissue and everything else. What’s great about someone’s DNA it does not ever change throughout their life. DNA was first used as a way of finding out paternity so
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DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. A molecule of DNA is made up of long chains of polymers and monomers called nucleotides. Those chains‚ two in particular that compose a strain of DNA‚ are formed by the grouping of nucleotides into polynucleotides. A nitrogenous base‚ a sugar‚ and a phosphate group make up the composition of a nucleotide. In the case of DNA‚ the four nucleotides that are found along the chain of DNA are thymine (T)
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