Extraction of caffeine from tea bags ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer was dried
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America Today It seems as though the number of Americans picking up drinking has increased through out the years. It use to be you hardly heard of people drinking. If they did it did not in volve as much alcohol as Americas drink now. Today in America there are five different types of alcohol drinkers which are: The Social Drinker‚ The Irresponsible Drinker‚ The Binge drinker‚ The Under aged drinker and The flat out Alcoholic. The Social Drinkers are ones who only drink on special occasions
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History of DNA WebQuest 1. Friedrich (Fritz) Miescher http://www.dnai.org/timeline/index.html Find Miescher on the timeline and click on the bucket with the Red Cross to watch the animation. In 1869‚ he extracted a substance from white blood cells that he called nuclein. What do you think he was actually extracting? 2. Frederick Griffith http://biology.clc.uc.edu/courses/Bio104/dna.htm Frederick Griffith’s famous experiment was conducted in 1928. In his experiment‚
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DNA synthesis inhibitors Nucleic acid inhibitors are chemicals which inhibit the production of nucleic acids including both DNA and RNA. DNA and RNA inhibitors inhibit enzyme action in DNA replication in the same way as the topoisomerase inhibitors we discussed earlier. Topoisomerase inhibitors (1) (10) are chemicals which interfere with the enzymes that allow DNA strands to separate and to re-join‚ a process that is required for the division of bacteria and without which DNA cannot effectively
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA? DNA is composed of two polynucleotide strands wound together into a structure known as a double helix. Each nucleotide consists of a sugar base .Nucleotides form together creating a sugar phosphate backbone to each strand. There are three forms of DNA that differ significantly. The most common‚ B form‚ is the structure most people have heard of. It consists of the right
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Epigenome biomarkers a. DNA methylation In the broad sense‚ epigenetics can be seen as a bridge between the genotype and phenotype‚ where the final outcomes of a locus or a chromosome can be changed without altering the underlying DNA sequence (Goldberg et al. 2007)‚ while epigenomics aims to study the location and nature of the gemonic sequences that are epigenetically modified (Fazzari and Greally 2004). Usually‚ three mechanisms have been attributed for epigenetics – DNA methylation‚ histone modification
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isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine
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What role does DNA play in inheritance? - DNA is the genetic material of inheritance. Deoxyribonucleic acid (DNA) is the body’s instruction manual for making who you are. DNA is present in any living being. You receive one -half of your DNA from your money and one-half from you Father. People with light eyes tend to carry recessive alleles of the major gene and people with dark eyes tend to carry the dominant alleles. Genes are located on rodlike structures called chromosomes that are found in the
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Introduction In 2015 Congress passed the DNA Fingerprint Act‚ which required that‚ beginning January 1‚ 2009‚ any adult arrested for a federal crime provide a DNA sample. As of May 2013‚ 29 states‚ in addition to the federal government‚ have enacted arrestee DNA collection laws‚ which authorize collection of DNA following arrest or charging. A U.S. Supreme Court decision‚ Maryland v. King (2013)‚ upheld a Maryland state law that allowed for the warrantless collection of a DNA sample for those arrested for
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WHAT IS RECOMBINANT DNA So what is rDNA? It stands for Recombinant DNA. Before we get to the “r” part we need to understand DNA. DNA is the keeper of all the information needed to recreate an organism. All DNA is made up of a base consisting of sugar‚ phosphate and one nitrogen base. There are four nitrogen bases‚ adenine (A)‚ thymine (T)‚ guanine (G)‚ and cytosine (C).The nitrogen bases are found in pairs‚ with A & T and G & C paired together. The sequence of the nitrogen bases can be
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