Extraction of DNA from Calf or Hog Thymus/Isolation of Yeast RNA I. Abstract Nucleic acids may be divided into two groups RNA and DNA. DNA contains almost all the genetic information while RNA serves as the bridge between the DNA and proteins. Study of both DNA and RNA initially involves proper extraction/isolation. The storehouse of eukaryotic DNA is the nucleus (and in the mitochondria)‚ so experimentally‚ DNA is extracted from tissues that have a high nuclear to cytoplasmic mass ratio‚ such
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Long stands of double helical DNA can fit into the nucleus of a single cell because DNA is specially packaged through a series of compaction events to fit easily within cell nuclei. Even though the length of DNA per cell is about 100‚000 times as long as the cell itself‚ it only takes up only about 10 percent of the cell’s volume. The DNA molecule‚ in order to condense‚ wraps itself around groups of histone proteins‚ and then the chromatin folds back on it‚ nucelosomes pack together to create a compact
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Effect of pH on Green Pea Germination Objective: To determine how pH affects the germination of green peas and to examine the degree of germination within each pH level. We will do this by using various buffer solutions (along with distilled water) to grow green peas. Hypothesis: We expected that the group of peas that encountered a pH of 7 to have the most and highest degree of germination. Materials: tap water buffer solutions pH 3‚5‚ 7‚ 9‚11 distilled
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Write a 350 to 700 word summary describing if the collection of DNA without consent unreasonably intrudes on an arrestees’ expectation of privacy. How long can police keep your DNA on file after an arrest or conviction? Can law enforcement use a person’s DNA to match against other crimes unrelated to the one they initially obtained it for? Provide examples and or reasons. The collection of DNA without consent can unreasonably be seen as intruding on someone who has been not been arrested however
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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Distillation Introduction: The recrystallization technique you learned last lab is a method for purifying solids. This week we will learn how to perform a distillation‚ a method for purifying liquids. Distillation is a common wet-chemical technique for separating organic compounds based on differences in boiling points. Upon heating a mixture of organic compounds‚ the more volatile compounds (those with the lowest boiling point) will vaporize first (i.e. be converted to gases)‚ leaving the higher-boiling
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Introduction When extracting lipids or fats from foods‚ both the method as well as the solvents chosen to perform a complete‚ or close to complete extraction are important. If these two elements are not taken into consideration‚ the extraction may not be complete‚ or the extract may contain a large quantity of undesired impurities. The natural fats and oil are mixtures of glycerides of fatty acids. Fats and oils are naturally occurring organic compounds which belong to a large group of water insoluble
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Extraction of Caffeine from Tea leaves Casinabe‚ Antonio Karlo‚*Catahay‚ Jesus Alfonso‚ Cerdena‚ O’neil‚ Cordova‚ Robelle Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España Street‚ Manila 108 Group 4 2MB Date Submitted: December 13‚ 2012 ------------------------------------------------- ABSTRACT: The purpose of this experiment is to extract the amount of caffeine present in tea bags. In this experiment‚ isolation‚ characterization and purification
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1.0 TITLE Extraction of Phenol and Flavanoid compound of the edible brown seaweed species (Eucheuma Cottonii). 2.0 INTRODUCTION 2.1 An overview of Seaweed Macro-algae or “seaweeds” are multicellular plants growing in salt or fresh water. They belong to the lower plants‚ meaning that they do not have roots‚ stems and leaves. Instead they are composed of a thallus (leaf-like) and sometimes a stem and a foot (holdfast). Some species have gas-filled structures to provide buoyancy. They are often
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Discussion: Extraction and crystallization- The purpose of this lab was to extract and crystallize compounds in a mixture using acids and bases. 1 gram of the unknown was dissolved into 30 mL of ethyl acetate. The mixture was then transferred to a separatory funnel. Using aqueous solutions of different pH we were able to separate organic acids‚ bases and neutral compounds from each other. Using three 10 mL portions of HCl‚ we reacted the HCl with the base in solution. This made it possible to
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