Introduction DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names‚ each representing the phases that it undergoes (chromosomes‚ chromatin‚ genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase‚ so that mitosis can make new cells‚ repairing and allowing for growth in the body. It is
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively
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Extracting DNA from Wheat Germ Cells Criteria to be assessed CE Introduction: DNA is the abbreviation for deoxyribonucleic acid. DNA is found in the nucleus of every cell & it stores the information that makes up living organisms. It is a double helix of 4 nucleotides. It contains a code that allows the body to make up proteins. The nucleotides are Cytosine‚ Guanine‚ Adenine and Thymine. (1) Wheat germ comes from wheat seeds. The ’ germ ’ is the embryo‚ which is the part of
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Extraction of DNA Molecule of an Onion In an Aqueous Solution By: Introduction DNA(deoxyribonucleic acid) is found in every living organism. It provides the information and instructions to build and regulate cells in organisms. The information it contains is used when organisms are being reproduced. It is made up of two polynucleotide chains known as DNA strands. Within the two chains there are four nucleotide units: adenine‚ thymine‚ cytosine‚ and guanine. Materials and Methods An onion
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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LABORATORY REPORT BIOLOGY CELL AND GENETICS (SB10203) TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the
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patient. However‚ we are only interested in the patient’s head and more specifically the brain‚ that is why in this step we perform an orientation step to orient all the brain images into a common coordination and then we perform the brain extraction using brain extraction tool (BET) which will result two images‚ one image contains only the brain and the other one contains the brain and the skull‚ both images are needed for further analysis‚ Figure 2.4 and Figure 2.5 Show the MRI image before and after
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