Electrophoresis and DNA fingerprinting Jani Lynette Hagen October 31‚2014 U74644799 Electrophoresis is a technique which uses an electric field to separate molecules‚ allowing for identification and characterization of the molecules. It is commonly used to separate nucleic acids and protein molecules of various sizes. To prepare the gel for electrophoresis the amount of agrose needed must be calculated. For a 0.8 percent gel 0.8 grams of agrose is necessary per 100 ml of buffer. The DNA fingerprinting
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MULTIPLE EXTRACTION OF CAFFEINE FROM DRIED TEA LEAVES USING DICHLOROMETHANE Delos Reyes‚ K.‚ Dizon‚ G.J.‚ Enriquez‚ J.R.‚ Estrada‚ G. and Garcia C. Group 4 2G Med Tech Organic Chemistry Laboratory ABSTRACT Caffeine was extracted from dried tea leaves by multiple extraction technique. 10g of tea leaves was boiled in a solution of 4.4 g anhydrous sodium carbonate and 100ml distilled water and was extracted three times using 20 ml of dichloromethane. Theresidue was collected by decanting
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1:38:46 PM Five “discovery skills” separate true innovators from the rest of us. | by Jeffrey H. Dyer‚ Hal B. Gregersen‚ and Clayton M. Christensen The Innovator’s DNA hbr.org 1692 Dec09 Dyer Layout.indd 61 | December 2009 | Harvard Business Review 61 11/2/09 1:39:02 PM Innovation SPOTLIGHT ON INNOVATION The Innovator’s DNA “How do I find INNOVATIVE PEOPLE for my organization? And how can I become more innovative myself?” These are questions that stump senior executives‚ who understand
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Radioisotopic labels would be used in experiments to identify semi-conservative replication in prokaryotes. Because we anticipated that a labeled DNA would have different density with unlabeled‚ which means‚ by analyzing the different density of DNAs‚ we can determine which of DNA is labeled‚ half-labeled or unlabeled. To this end‚ I will use c13 label the bacteria and abruptly change carbon source with C12. Then I will collect four samples in different time and analyze the results from centrifugal
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QUIZ 02 ECO402 Quiz # 2 ECO402 (Microeconomics) Semester Spring 2008 Total Marks 15 Instructions: 1. 2. 3. 4. 5. This quiz covers Lesson 14-17. Last date for submission of quiz is 12/05/08. Upload your quiz with in due date and time. No quiz will be accepted after due date and time. In MCQs‚ write only one option in the answer sheet which you think is correct. More than one answer will be marked zero. 6. No quiz will be accepted through e-mail. 7. Please submit your solution
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www.hbrreprints.org Decoding the DNA of the Toyota Production System by Steven Spear and H. Kent Bowen Included with this full-text Harvard Business Review article: The Idea in Brief—the core idea The Idea in Practice—putting the idea to work 1 Article Summary 2 Decoding the DNA of the Toyota Production System A list of related materials‚ with annotations to guide further exploration of the article’s ideas and applications 12 Further Reading The Toyota story has been intensively
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Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided‚ and then recrystallize the caffeine. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). Anhydrous calcium chloride pellets were used to dry the solution and emulsion layer and the DCM was then decanted. After washing the anhydrous calcium chloride pellets with more DCM‚ the solvent was evaporated‚ leaving
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…….asked us to create a stylised performance using the narrator’s text; DNA by Dennis Kelly. We‚ had to use still images‚ mime and movement and a section from to play to be delivered in response to Stanislavski. We stayed our performance by having …… and …. as the two naturalistic performers‚ sitting side by side on the floor‚ quite far apart from each other to show the audience the lack of closeness between…. and …. Leah being the main narrator‚ sat up on her knees‚ inviting the audience in to listen
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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generation and the use of DNA information and analyses will contribute greatly to the field of criminal investigation and in effect‚ downgrade with expediency the crime rate in the country‚" Angara said. What are those for? DNA matching will become an ever more powerful weapon against crime. Law enforcement will increasingly be able to identify suspects from biological evidence at crime scenes‚ saving investigative time and protecting innocent people from suspicion. When DNA evidence is properly handled
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