the structure of DNA and discuss how it facilitates the ability of DNA to act as genetic material • Compare and contrast prokaryotic and eukaryotic chromosomes • Describe the process of DNA replication as a semi-conservative process. Compare and contrast the synthesis of the leading and lagging strands in DNA replication. • Explain how the genotype of an organism determines its phenotype. • State the central dogma of genetics and explain the roles of DNA and RNA in polypeptide
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DNA fingerprinting is a way of identifying a specific individual‚ rather than simply identifying a species or some particular trait. It is also known as genetic fingerprinting or DNA profiling. As a technology‚ it has been around since at least 1985‚ when it was announced by its inventor‚ Sir Alec Jeffreys. DNA fingerprinting is currently used both for identifying paternity or maternity and for identifying criminals or victims. There is discussion of using DNA fingerprinting as a sort of personal
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DNA sequencing From Wikipedia‚ the free encyclopedia [pic] The term DNA sequencing refers to sequencing methods for determining the order of the nucleotide bases—adenine‚ guanine‚ cytosine‚ and thymine—in a molecule of DNA. Knowledge of DNA sequences has become indispensable for basic biological research‚ other research branches utilizing DNA sequencing‚ and in numerous applied fields such as diagnostic‚ biotechnology‚ forensic biology and biologicalsystematics. The advent of DNA sequencing
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ions -DNA replication minimizes errors such as the DNA that is duplicated but changes in the DNA do occur‚ producing mutations. Although most mutations are either neutral or harmful they are also the raw material for evolution. Such mutations from alleles‚ alternate forms of a given gene that may produce differences in structure or function such as black‚ brown or blond hair in humans‚ or different mating calls in frogs. Stages of Mitosis~ 1)Parent cell. 2)Chromosomes make identical copies
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DNA FINGERPRINTING Background Reading - Nelson Biology and Campbell Biology Purpose - To understand the basics of DNA fingerprinting used in the Canadian courts for crime convictions and paternity suits. Introduction The process of DNA fingerprinting was developed by Professor Alec Jeffreys at Leicester University in 1984 as a form of genetic analysis. It was first used in the law courts of England in 1987 to convict a man in a rape case. It has now been used successfully in many crime and paternity
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DNA REPLICATION At the replication origin DNA helicase attaches to a strand of DNA and begins to break apart hydrogen bonds in order to unravel a section of the double helix. The section of DNA that is unwound is called the replication bubble and the “Y” shaped sections are called the replication forks. In order to stop the unwound section from binding back together‚ single strand binding proteins react with the single strand portions on the DNA causing them to stay separated. Although the leading
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An Introduction to DNA microarrays Rebecca Fry‚ Ph.D. http://www.buffalo.edu/UBT/UBT What is a DNA Microarray? genes or gene fragments attached to a substrate (glass) Tens of thousands of spots Hybridized slide Two dyes Image analyzed 1 The Beginnings of Microarray Technology Lockhart et al.‚ 1996 Nature Biotechnology “Expression monitoring by hybridisation to high-density oligonucleotide arrays” Schena et al.‚ 1995 Science “Quantitative monitoring of gene expression
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DNA replication is a biological process that occurs in all living organisms and copies their DNA. DNA replication during mitosis is the basis for biological inheritance. The process of DNA replication starts when one double-stranded DNA molecule produces two identical copies of the molecule. Each strand of the original double-stranded DNA molecule serves as template for the production of the complementary strand‚ a process referred to as semiconservative replication. Cellular proofreading and error-checking
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2014/15 Module name and number Molecular Biology & Genetic Engineering: BS941 Assignment title “DNA sequencing: where we are and where it’s going” Student Number 1464986 Word Count 2310 The article focuses on the advances achieved in DNA sequencing by first providing a brief background on DNA‚ and how it was initially sequenced. The paper then takes into consideration four of the major DNA sequencing techniques. These include: Sanger’s Chain Termination Method‚ Pyrosequencing‚ Single Molecule
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DNA helicase -separates strands of nuclei acid‚ breaks H bond between nitrogenous bases.‚ works at the replication fork -DNA PRIMASE- lays RNA primer ‚ acts as new strand‚ can only add nucleotides to a free3’ end ‚ lays nucleotide with a 5’ orientation -DNA POLYMERASE 3- adds nucleotides using base pair rules lcreating 2 new daughter strands‚ only adds to a free 3’end and lays down nucleotide with 5’ orientation. Pol3 continuously synthesizes new daughter cell(leading strand) same direction as
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