lab assistant or instructor. Consider that the nucleophiles may be influenced by interactions with the solvent. These interactions are much more significant in protic solvents (e.g.‚ water and alcohol) than in aprotic solvents (e.g.‚ acetone or DMSO). Experimental Procedure
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INTRODUCTION An important aspect of any biotechnological processes is the culture of animal cells in artificial media. These animal cells in culture are used in recombinant DNA technology‚ genetic manipulations and in a variety of industrial processes. Now-a -days it has become possible to use the cell and tissue culture in the areas of research which have a potential for economic value and commercialization. The animal cell cultures are being extensively used in production of vaccines‚ monoclonal
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Yellow colored‚ odorless crystalline powder (Table 5.1). 5.2 DRUG IDENTIFICATION Amphotericin B was identified by the procedures described in British Pharmacopoeia 2003. 5.2.1 Chemical Test In a drug solution (1 mL of 0.05% w/v) in dimethyl sulfoxide (DMSO)‚ 5 mL of orthophosphoric acid was added. A blue ring was observed at the
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They also discovered that less time was needed to achieve maximum adsorption with SnS50+I than the SnSg+I and SnSp+I. The reason for this was not discovered. Although another worthy note from this experiment is that originally the PAN was dissolved in DMSO before it was added to the chalcogel. This actually improved the final surface area of the resulting hybrid sorbent. This leaves this notion also up for further investigation and
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Journal of Biological Research-Thessaloniki 16: 194 – 201‚ 2011 J. Biol. Res.-Thessalon. is available online at http://www.jbr.gr Indexed in: WoS (Web of Science‚ ISI Thomson)‚ SCOPUS‚ CAS (Chemical Abstracts Service) and DOAJ (Directory of Open Access Journals) Mesenchymal cells isolation from Wharton’s jelly‚ in perspective to clinical applications Iro KOLIAKOS 1*‚ Nikos TSAGIAS 2 and Vassilis KARAGIANNIS 2 1 2 Stem Cells Bank‚ National Research Foundation‚ Athens‚ Greece C Gynecology
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Analysis of the RBM22 Protein Association with Gene Expression through Affinity Tagging Lucas Akin Stream Faculty: Scott Stevens Research Professor: Albert MacKrell University of Texas at Austin School of Biological Sciences Abstract Affinity tagging of proteins is an important method for biochemical analyses [4] and has proven effective in further expanding our knowledge of their specific functions. A group of interactive proteins expressed by bacteriophage ʎ known as exo‚ bet‚ and
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pressure limit in microwave. To overcome the pressure problem‚ a base potassium tert-butoxide was added to dicyclopentadiene in diglyme and microwaved for 10 in @ 185oC to obtain cyclopentadienyl anion. This anion was added to a solution FeCl2.4H2O in DMSO and ferrocene was obtained in 5 minutes with a yield of 86%. To further
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0.5 min * Loop size: 1ml * GC Cycle time: 37 min Standards and sample preparation _ Class 1 standard reference solution: Transfer 1 ml of the USP reference standard for residual solvent class 1 to a 100 ml volumetric flask. Add 9 ml of DMSO. Dilute to volume with water and agitate. Transfer 1 ml of this solution to a 100 ml volumetric flask‚ dilute to volume and agitate. Transfer 10.0ml of this solution to a 50ml volumetric flask dilute to volume and agitate. _ Class 1 residual solvents
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ABSTRACT Purpose: To evaluate the antifungal and cytotoxicity activity of four medicinal plants like Mystroxylon aethiopicum‚ Lonchocarpus capassa‚ Albizia anthelmentica and Myrica salicifolia. Methods: Microdilution method and brine shrimp lethality test were employed to evaluate antifungal and cytotoxicity of plant extracts. Results: Lonchocarpus capassa leaf extracts exhibited antifungal activity against tested fungal strains with MIC range of 0.78 – 3.125 mg/mL with LCLA extract inhibiting C
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VOL. 6‚ NO. 9‚ SEPTEMBER 2011 ARPN Journal of Agricultural and Biological Science ©2006-2011 Asian Research Publishing Network (ARPN). All rights reserved. ISSN 1990-6145 www.arpnjournals.com PROMISING ANTIFUNGAL EFFECT OF SOME FOLKLORIC MEDICINAL PLANTS COLLECTED FROM EL- HAMMAM HABITAT‚ EGYPT AGAINST DANGEROUS PATHOGENIC AND TOXINOGENIC FUNGI Department of Bioprocess Development‚ Genetic Engineering and Biotechnology Research Institute‚ Mubarak City for Scientific Research and Applied Technology
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