recrystallized in aqueous EtOH (70%) to give pure products. Selected spectra: 3 -(2′-benzothiazolo)-2‚3-dihydro-2-(4-chlorophenyl)-quinazolin-4(1H)-ones ( Table 1 Entry 1): m.p 229-239 oC; IR (KBr): υ = 3335‚ 1634‚ 1502‚ 1406‚ 1254‚ 740cm-1; 1H NMR (400 MHz‚ DMSO-d6): δ = 6. 81 (t‚ 1H‚ J = 8 Hz)‚ 6.85 (d‚ 1H‚ J = 8 Hz)‚ 7.30 (d‚ 2H‚ J = 8 Hz)‚ 7.36 (t‚ 3H‚ J = 8 Hz)‚
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conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe that both types of treated cells will express the beta-actin gene because it is a housekeeping gene. However‚ only HL-60 cells treated with PMA will express the MMP-9 gene by their RNA. The purpose of this lab is to view the effects of differentiation on protein synthesis in PMA and DMSO treated cells‚ as well as in untreated
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Prostate Cancer is the second most common cancer among men‚ after skin cancer‚ and is the leading cause of cancer death in men in the United States. Prostate cancer is caused by changes in the DNA of a prostate cell that turns off tumor suppressor genes and activate oncogenes. Essentially‚ cancer is an epigenetic programming problem. Epigenetics is the study of trait variations that are caused by factors that turn genes on and off and change how cells read genes. Immune evasion is when alterations
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sequential extraction. Solvents; Hexane‚ Chloroform‚ Acetone and Methanol were evaporated by placing the extracts in fume hood for 7-10 days. Stock solution prepration: To prepare 40 µg/µl stock concentration in Dimethyl sulfoxide (DMSO)‚ 40mg of each plant extract was suspended in DMSO‚ inside Laminar flow hood. Stock solutions were filtered through 0.22 µm filter and then stored at -20ᵒC.
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Its 1H-NMR (DMSO-d6) δ ppm spectrum (chart 6) revealed the presence of these signals at δ 3.8 (s‚ 3H‚ OCH3)‚ 6.57 (s‚ 1H‚ H-5 pyridone)‚ 7.08 (d‚ J = 8.4 Hz‚ 2H‚ aromatic)‚ 7.18 (m‚ 2H‚ indolyl)‚ 7.49 (d‚ J = 8.5 Hz‚ 1H‚ indolyl)‚ 7.69 (d‚ J = 8.4 Hz‚ 2H‚ aromatic)‚ 7.86 (d‚ J = 8.5 Hz‚ 1H‚ indolyl)‚ 8.27 (d‚ 1H‚ indolyl)‚ 12.06 (s‚ 1H‚ NH pyridone‚ D2O exchangeable)‚ 12.35 (1H‚ d‚ NH indole‚ D2O exchangeable). The 13C-NMR (DMSO-d6) δ ppm (chart 7) revealed the existence
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Infrared was recorded on a Perkin-Elmer 1650 FT-IR spectrophotometer‚ using a sample in KBr disks. 1H NMR and 13C NMR spectra were recorded on a Varian Gemini (300 MHZ)‚ using TMS as an internal standard and DMSO-d6 as solvent. Mass spectra were taken on Shimadzu GCMS QP-1000EX instrument by direct inlet technique‚ at beam energy 70 eV. The 3-acetylquinolinone AQ was prepared according to literature method (Roscheger and Stadlbauer‚ 1990). 2-(1-Methyl-4-hydroxy-2-oxo-1
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six pi electrons binds every carbon equally to the metal forming a sandwich type structure. Ferrocene has the properties of both an activated benzene (undergoes electrophilic substitution reactions) and a ferrous ion (oxidation reaction). Glyme and DMSO are used as solvents as they have moderate polarity so they would break up KOH into K+ and OH-‚ without breaking the hydrogen bonding in the hydroxide ion. Cyclopentadiene is relatively acidic so a strong base would be needed to abstract
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Chemicals Hordenine and methyl jasmonate were purchased from Tokyo Chemical Industry (Tokyo‚ Japan). Dimethyl sulfoxide (DMSO) was obtained from Sigma-Aldrich (Tokyo‚ Japan). Plant materials Two-year-old seedlings of Vitis vinifera cv. Koshu were cultivated in perlite:vermiculite (1:1) soil in a growth chamber (11.8 Wm-2 for 14 h in a day) at 27 °C. Seedlings of Fragaria×ananassa cv. Nyoho were grown in 55% peat moss‚ 10% perlite‚ 5% vermiculite‚ and 30% decomposed granite soil at 25 ºC in a greenhouse
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Article pubs.acs.org/jmc Thiazolopyridine Ureas as Novel Antitubercular Agents Acting through Inhibition of DNA Gyrase B Manoj G. Kale‚† Anandkumar Raichurkar‚†‚∇ Shahul Hameed P‚†‚∇ David Waterson‚†‚○ David McKinney‚∥ M. R. Manjunatha‚† Usha Kranthi‚† Krishna Koushik‚† Lalit kumar Jena‚† Vikas Shinde‚† Suresh Rudrapatna‚† Shubhada Barde‚† Vaishali Humnabadkar‚‡ Prashanti Madhavapeddi‚‡ Halesha Basavarajappa‚‡ Anirban Ghosh‚‡ VK Ramya‚‡ Supreeth Guptha‚‡ Sreevalli Sharma‚‡ Prakash Vachaspati
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recorded in DMSO on a Thermo UV1 spectrophotometer. IR spectra were recorded on a Perkin Elmer Spectrum SP-2 Fourier transform spectrophotometer using KBr pellets (4000–400 cm−1). 1H and 13C NMR spectra were recorded on a Bruker Avance II (400 MHz) FTNMR spectrometer using DMSO-d6 as solvent at 400 MHz and 100 MHz‚ respectively. TMS was used as internal reference for 1H NMR and 13C NMR. The 119SnNMRspectra with proton noise decoupling were recorded on a Bruker Avance II spectrometer using dry DMSO as the
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