Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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As mentioned above‚ bacterial growth rates during the phase of exponential growth‚ under standard nutritional conditions (culture medium‚ temperature‚ pH‚ etc.)‚ define the bacterium’s generation time. Generation times for bacteria vary from about 12 minutes to 24 hours or more. The generation time for E. coli in the laboratory is 15-20 minutes‚ but in the intestinal tract‚ the coliform’s generation time is estimated to be 12-24 hours. For most known bacteria that can be cultured‚ generation times
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Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how to accurately use it to view specimens. The staining method was used
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BUSINESS MATH Simple Interest and Simple Discount Business Math‚ Eighth Edition Cleaves/Hobbs © 2009 Pearson Education‚ Inc. Upper Saddle River‚ NJ 07458 All Rights Reserved 11.1 The Simple Interest Formula Find simple interest by using the simple interest formula. Find the maturity of a loan. Convert months to a fractional or decimal part of the year. Find the principal‚ rate or time using the simple interest formula. Business Math‚ Eighth Edition Cleaves/Hobbs © 2009 Pearson
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan
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Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
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FUTURE SIMPLE OR PRESENT SIMPLE A. 1. I (go) and see Venice as soon as I (reach) Italy. 2. If the sun (be) red‚ it is a sign that we (have) a fine day tomorrow. 3. If you (not be) at home by six‚ I (eat) without you. 4. If Mother (not go) to the market early‚ she (not get) fresh fish. 5. If Adam (sell) his car he (afford) a holiday in Florida. 6. Take some coins in case you (need) to phone. 7. If nothing (go wrong)‚ they (deliver) the furniture today. 8. Unless you (change) your mind we (not
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Simple Machines Definitions: Machine- A device that makes work easier by changing the speed ‚ direction‚ or amount of a force. Simple Machine- A device that performs work with only one movement. Simple machines include lever‚ wheel and axle‚ inclined plane‚ screw‚ and wedge. Ideal Mechanical Advantage (IMA)- A machine in which work in equals work out; such a machine would be frictionless and a 100% efficient IMA= De/Dr Actual Mechanical Advantage (AMA)- It is pretty much the opposite of
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I. Abstract: This experiment tests two parameters‚ deflection and strain‚ associated with a simple truss subjected to a loading at its bottom end as would be the case if a constructed bridge was subjected to loadings by cars‚ trucks‚ trailers‚ pedestrians‚ etc. The set up contained an instrumented truss mounted in a test frame with strain gauges attached to the center of each truss member and a linear potentiometer displacement transducer (LPDT) placed underneath the truss assembly to measure displacement
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