Determination of Total Carbohydrateby Anthrone Method Carbohydrates are the important components of storage and structural materials in the plants.They exist as free sugars and polysaccharides. The basic units of carbohydrates are themonosaccharides which cannot be split by hydrolysis into more simpler sugars. The carbohy-drate content can be measured by hydrolysing the polysaccharides into simple sugars by acidhydrolysis and estimating the resultant monosaccharides. PRINCIPLE Carbohydrates are
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IODINE TEST FOR STARCH The Iodine test is used to test for the presence of starch. Iodine solution — iodine dissolved in an aqueous solution of potassium iodide — reacts with the starch producing a purple black color. The colour can be detected visually with concentrations of iodine as low as 0.00002M at 20°C. However the intensity of the colour decreases with increasing temperature and with the presence of water-miscible‚ organic solvents such as ethanol. Also the test cannot be done at very low
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through cell walls in our body into the blood stream. It has to be the correct enzyme - amylase is the only enzyme that will break down starch. Starch is a polymer composed of a series of glucose monomers. Amylase hydrolyzes starch into glucose and disaccharide maltose (Lab pg62). The lock and key theory is a possible explanation of why‚ only one type of enzyme can breakdown starch. The analogy is that‚ the enzyme is the lock and the key is the substrate‚ only the correct key (substrate) fits into the
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Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict’s test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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Saccharification of Pineapple Ananas comosus peelings through Dilute Acid Hydrolysis Rhoy M. Capul Ma. Michelle Carmel O. Aquino Alia Jenine S. Caceres San Pablo City National High School San Pablo City Mrs. Glory M. Serrano Research Adviser A Science Investigatory Project in fulfillment of Research project S.Y. 2010-2011 Abstract The pineapple is one of the leading products in the Philippines‚ as it thrives in tropical climates. However
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3.1 Proteins Table 3.2 Biuret Test Tube Contents Final Color Conclusions 1 Distilled water Transparent‚ light blue‚ navy Possibly little protein with clear peptide or no protein at all 2 Albumin Dark Purple Proteins are present with purple peptides 3 Pepsin Purplish blue‚ darkish blue Proteins are present with purple or black colored peptides 4 Starch Light blue‚ really clear Possible little protein with clear peptide or no protein at all Our results are correct because water
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Tittle : Identification of Biochemicals in Their Pure Form Objective : To identify the component of the solution in its pure form with various food tests and state the justifications. Apparatus : Test-tube ‚ test-tube holder ‚ water bath 95’c ‚ measuring cylinder ‚ white tile ‚ test-tube rack . Materials : Glucose‚ sucrose ‚ fructose ‚ hydrochloric acid ‚ potassium hydroxide ‚ albumin ‚ cooked starch ‚ corn oil ‚ copper (ll) sulphate solution ‚ ascorbic acid solution ‚ DCPIP solution ‚ Millon’s
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Practical 1 : Carbohydrates Introduction : Carbohydrates are important to human life ‚ this practical is to investigate physical ‚ functional and sensual properties of different carbohydrates ‚ including monosaccharides and disaccharides . Experiment 1 Title : Relative Solubility of Carbohydrates ‚ Glucose ‚ Galactose and Lactose . Objective : To determine the relative solubility of glucose ‚ galactose and lactose by measuring their saturation point at the same constant temperature.
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Intro: Carbohydrate‚ any of a large group of compounds in which hydrogen and oxygen‚ in the proportions in which they exist in water‚ are combined with carbon; the formula of most of these compounds may be expressed as Cn(H2O)n. Structurally‚ however‚ these compounds are not hydrates of carbon‚ as the formula would seem to indicate. Carbohydrates‚ as a class‚ are the most abundant organic compounds found in nature. They are produced by green plants and by bacteria using the process known as photosynthesis
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