to Volume ratio in Agar Introduction: What is an efficient way to maximize mass but minimize diffusion time in cell? Answer: An efficient way to maximize mass but minimize diffusion time in a cell is to increase its surface area. If you increase the surface area of a cell relatively to its volume‚ then the diffusion time will decrease. Materials: Agar cubes‚ bromothymol blue- pH indicator‚ vinegar‚ ruler‚ spatula‚ beaker. Methods: I used agar cubes as cell models. The agar was cut into blocks
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Diffusion is one of several transport phenomena that occur in nature. A distinguishing feature of diffusion is that it results in mixing or mass transport‚ without requiring bulk motion. Thus‚ diffusion should not be confused with convection‚ or advections‚ which are other transport mechanisms that utilize bulk motion to move particles from one place to another. In Latin‚ "diffundere" means "to spread out". There are two ways to introduce the notion of diffusion: either a phenomenological approach
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The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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For this experiment you will need 39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5
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simple diffusion and understand how to make % solutions. Exercise 2 demonstrated the principle of osmosis: observing the movement of water by osmosis from an area of lower concentration of a solute to an area of higher concentration of a solute. Materials and Method: Refer to pages 47-51 of Bio 203L Lab Manual. Results/ Discussion: The effect of the chloride ion on its rate of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride
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Factors Affecting Diffusion How does temperature affect diffusion? What is Diffusion? Definition 1: Diffusion is a process where by molecules move from an area of high concentration area to an area of low concentration. Definition 2: Diffusion is an evidence for moving particles. The spreading out of a gas is called diffusion and it takes place in haphazard and random way. Gases diffuse because the particles collide with other particles and bounce of in all directions. For example when
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DIFFUSION REVIEW WORKSHEET 1. Define diffusion and what causes it to occur? The action of spreading the light from a light source evenly so as to reduce glare and harsh shadows. Diffusion is caused when entities are small and moving in molecules. They collide because they are moving at a random pace. The temperature causes diffusion. 2. Define the terms hypertonic‚ hypotonic‚ and isotonic solution. * Hypertonic: Having increased pressure or tone‚ in particular. * Hypotonic:
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