proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during
Premium Staining Gram staining
Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
Premium Staining Bacteria Gram staining
The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
Premium Gram staining Staining Microbiology
Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
Premium Gram staining Bacteria Staining
Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
Premium Bacteria Adenosine triphosphate
Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
Premium Escherichia coli Staining Gram staining
purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to
Premium Gram staining Staining Microbiology
Lab Report – Strong Example Problem/Question Does using a base coat before painting nails prevent chipping? Background Information When painting nails‚ some manicurists use a base coat before applying the polish. The purpose of a base coat is to help the polish stick to your nails more easily. Base coats also help to protect your natural nail from the staining effects of colored polishes. Ridge-filling base coats fill in the grooves and cracks in the nail to create a smoother appearance
Premium Left-handedness French Revolution Handedness
OCEAN COUNTY COLLEGE OFFICIAL COURSE DESCRIPTION DEPARTMENT OF SCIENCE AND ENGINEERING 1. Course Number and Title: CHEM-181 General Chemistry I 2. Semester Hours: 4 Contact Hours: (3 + 2) Lecture Lab 3. Catalog Description This course‚ intended for science majors‚ is the first course of a two-course sequence. Course topics include stoichiometry‚ inorganic nomenclature‚ solutions‚ gas laws‚ thermochemistry‚ atomic structure‚ and chemical bonding. The laboratory work includes
Premium Chemistry Molecule Atom
Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
Free Bacteria Staining Gram staining