Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan
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MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
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Differential Staining Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each‚ learn and employ the streak and pour dish techniques‚ and generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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Cellular and Molecular Techniques - Lab #1 BIO 349 Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how
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Site Name: PhysioEx 8.0Author Name: Stabler‚ ZaoLocation on Site: Exercise > Lab Quiz 100% Correct of 20 items:20 Correct: 100%0 Incorrect: 0% Submitted on Sun Sep 27 2009 15:44:17 GMT-0600 (Mountain Daylight Time) --------------------------------------------------------------------------Question 1 Differential permeability can also be described as: Correct: the allowance of only certain substances to pass through a membrane without hindrance. Compare the molecule size and the MWCO for the
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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