Determination of the concentration of vitamin C in fruits/vegetables by using the DCPIP test (2008) Principle : Vitamin C is a strong reducing agent. It can decolorize the blue dye DCPIP. The amount of vitamin C in a food sample can be estimated by the amount of it that is used to decolorize a fixed amount of DCPIP. Apparatus : Test tube‚ clean and dry Test tube rack 5 ml syringe or a good quality squeezing pipette 1 ml pipette
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Measuring the Vitamin C content in a variety of fruit juices Background Information Vitamin C‚ also called ascorbic acid‚ is a water soluble vitamin. It is a powerful reducing agent that is able to decolourise blue DCPIP (2.6-dichlorophenol indophenols). The decolourisation of DCPIP can therefore be used to calculate the vitamin C content of a variety of fruit juices. Research Question – To determine how the content of Vitamin C (%) will differ in fresh fruits (orange‚ mango‚ lemon‚ lime‚ blackcurrant
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Determination of Vitamin C by an Iodometric Titration Purpose: The goal of this lab is to determine the concentration of vitamin C in juices and Real Lemon. A redox titration‚ involving an iodometric method‚ will be used to do the analysis. The samples will be classified by their Vitamin C content. Introduction: Although most mammals can synthesize vitamin C‚ or ascorbic acid (C6H8O6)‚ from sugars‚ man must ingest considerable quantities of this substance. The National Academy of Sciences recommends
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the Vitamin C from a rival company and compare with that of my company and find out the best value for the money spent by the consumer. Controlled Variable * Mass of the tablet and hereby vitamin C in rival company tablets * Consistency of components in the Vitamin C tablet * Concentration of NaOH Apparatus 1. Burette (50ml) 2. Conical flask (50ml) 3. Vitamin C tablet (1 tablet per one trial) 4. Sodium Hydroxide (NaOH) (500cm3) a. The NaOH concentration in
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Effect of Heat on Vitamin C Objective: - To determine whether the concentration of Vitamin C in a fruit juice changes upon heating at a constant temperature for different periods of time. - To investigate how the trend goes (whether the concentration of Vitamin C increases or decreases) if the amount of Vitamin C does change. Theory: - Hypothesis: The concentration of Vitamin C in the fruit juice changes and decreases upon heatingat a constant temperature for increasing periods of time
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Abstract Vitamin C‚ also known as ascorbic acid‚ is a water soluble vitamin that is regarded as one of the safest and most effective nutrients. Vitamin C can be found in most fruits and vegetables. The goal of the experiment is to find out the concentration of vitamin C in three citrus fruits‚ orange‚ lemon and lime‚ and to compare them and find out which fruit contains the highest concentration of Vitamin C. By using the juice from each fruit to make a solution to combine with 10cm³ of DCPIP until
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Lab Report: MEASUREMENT OF VITAMIN C Introduction Nutritional diseases were once the number one cause of death only a century ago‚ but rarely affect our lives today. The complete lack of vitamin C (ascorbic acid) characterized by dark purple spots on the skin and rotting gums‚ a disease called scurvy‚ took the lives of many sailors up until the mid 1800s when the prevention was discovered. Consequently‚ British sailors became referred to as ‘limeys’ because of their daily habit of eating
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lemon fruit or orange fruit contain more vitamin C. Section B. Identifying variables 1. Independent variable Numbers of dropper of juice Time for the juice the decolorized 2. Dependent variable The concentration of DCPIP solution 3. Controlled variable The concentration of DCPIP solution The amount of fruit juice The temperature The time that the juice expose to the air Section C. Introduction 1. Background information DCPIP is an enzyme-catalyzed oxidation electron
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Hramar ( 7 ) Experiment 7 : Analysis of commercial vitamin C tablet Title: Determination of the vitamin C content (ascorbic acid) of a commercial vitamin C tablet and compare the result with the value specified by the manufacturer. Theory : In this experiment‚ we have to determine the vitamin C content of a commercial vitamin C tablet which is the mass of ascorbic acid in the tablet. As iodine is a weak oxidizing agent used mainly for the determination of strong reducing agents‚ ascorbic acid C6H8O6
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of two students required: * vitamin C indicator solution * three 50 mL beakers or medicine cups * one 10 mL graduated cylinder * three disposable pipettes * one stirring rod * Sources of Vitamin C: freshly squeezed OJ‚ bottled OJ‚ frozen OJ‚ canned OJ * Optional: container for waste solutions and source of clean rinse water There are two different preparations (starch-iodine or indophenol) that may be used for the vitamin C indicator solution. Neither is more
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