concentration of reducing sugars is by heating with 3‚5 dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration of unknown sample can then be read off a calibration curve(standard curve) created using known sugar concentrations.The dilutions of a solution of known concentration
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a Monoprotic Weak Acid Chemistry Lab 152 Professor: James Giles November 7‚ 2012 Abstract: The purpose of this experiment was to determine the pKa‚ Ka‚ and molar mass of an unknown acid (#14). The pKa was found to be 3.88‚ the Ka was found to be 1.318 x 10 -4‚ and the molar mass was found to be 171.9 g/mol. Introduction Acids differ considerable as to their strength. The difference between weak and strong acids can be as much
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Reviewer’s Name: Sanjeev Mishra UMN ID Number: 4585009 The Photoelectric Effect: A Determination of Planck’s constant Ian E. Jaeger School of Physics and Astronomy‚ University of Minnesota – Twin Cities 116 Church St. S.E.‚ Minneapolis‚ MN 55455 Abstract The photoelectric effect was explored to determine an experimental value of Planck’s constant‚ h. Included is a brief introduction to the history leading up to Einstein’s discovery of the photoelectric effect as well as the theory behind
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! ! ! ! Determination of Gas Constant R through Mg and HCl Reaction ! ! ! ! ! !1 of !9 IB Chemistry SL (Jr) Candidate: Yunha Kim Objective ! Candidates will react Magnesium with Hydrochloric acid and collect Hydrogen. The grams of the produced Hydrogen will be calculated and compared to the theoretical yield of Hydrogen. ! Controlled Variables ! Mass of Magnesium Ribbon This will be kept constant as the objective is to determine the Gas Constant (R). If this
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Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
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of copper sulfate using the spectronic 20. Four solutions of different concentrations of H₂O and CuSO₄ were put into the spectronic 20 to observe their absorption of light. Then‚ a graph was created to plot down the measurements. Using the calibration curve of the graph‚ the concentration of the unknown substance was found out to be .12M. Background/Introduction: Beer’s law (which states that by holding the pathlength of a particular substance- in this case‚ CUSO₄ - constant‚ the absorption of
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sugars by acid of carbohydrate cereals powder‚ jams (total sugar content) and jams (reducing sugar content) were performed in order to study reducing sugar production. The study involved hydrolysis carbohydrate cereals powder and jams by immersing in boiling water at environmental temperature. Acid hydrolysis was carried out through reflux boiling for 20 minutes with 10ml 0f 1.5M concentrations of diluted sulphuric acid. Reducing sugars concentrations were determined by spectrophotometry using the 3
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TITLE: Determination of Riboflavin by using UV-Vis spectrophotometry. ABSTRACT: The amount of Riboflavin in various brand of children multivitamins was determine by using UV-Vis spectroscopy instrument‚ the series of standard of riboflavin was prepared in order to determine the amount of the riboflavin in the sample of children multivitamins. The maximum amount of riboflavin was found in the Champs multivitamin that contains 0.1155 mg followed by Chewies 0.1131 mg and Scott’s 0.0672 mg. OBJECTIVES:
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the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a & b) are constants. In the equation‚ A = abc where A is the absorbance
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Determination of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96‚ and the pKa’s found experimentally were 2.0‚ 4.0‚ and 9.85. The accepted pKa values were found to be 2.10‚ 4.07‚ and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid‚
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