Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with
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1 Practical report: Determination of concentration using a standard curve Introduction Spectrophotometer is an instrument that confirms the measurement of specified wavelength of light that passes through medium. The measurement of light absorbance by a solution is also done with spectrophometer. Absorption spectrum Spectrometers are mainly and widely functional in identifying the components of solutions that helps determine the components’ concentrations. Compounds are experimentally identified
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Clinical Chemistry Manlangit‚ Joyce Ann U. EXPERIMENT Title: Standard Curve Preparation Objectives: 1. Properly set up a standard curve 2. Using the prepared curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add
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Albumin: Why is it ImPortant? Lauren Maister-Green‚ RD; Alisha Chasey‚ MS‚ RD; Beth Spanier‚ RD; Meredith Gilliatt-Wimberly‚ MS‚ RD TIPS . . . • At each meal try to include one of these high quality protein foods: chicken‚ turkey‚ fish‚ beef‚ pork‚ or eggs. n monthly lab reviews‚ dialysis patients may be told that their albumin level is low and they need to eat more protein. But what is albumin? Why is an albumin blood test important? What factors affect albumin? How can albumin levels be
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bell curve is literally‚ the symmetrical curve created on a graph when using a frequency distribution method for a set of data‚ splitting the mean symmetrically. There is a big difference between standard deviation and the bell curve! Standard deviation shows the difference in variation from the average; the bell curve‚ also normal distribution or Gaussian distribution‚ shows the standard deviation and is created by the normal or equal distribution of the mean among either half. The bell curve is an
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The Efficacy‚ Safety and Benefits of Bovine Somatotropin and Porcine Somatotropin Prepared for the American Council on Science and Health by Terry D. Etherton‚ Ph.D. Professor of Animal Nutrition and Physiology in the Department of Dairy and Animal Science at The Pennsylvania State University ACSH Publications Editor: Andrea Golaine Case‚ M.S. Please Note: For the web site version of this report‚ tables are provided but diagrams are not. Executive Summary As the world’s population grows
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whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion. Figure 1: Standard Curve of light absorbance by ten different concentrations of Methylene Blue. The data of the figure is collected by our group Mitochondria. The relationship between two
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LABORATORY REPORT PRACTICAL 2 (ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of
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Instructor: Dr. Muna Hassuneh. Report subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with
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