There are approximately 40‚000 enzymes living in one human cell‚ each responsible for a chemical reaction. Enzymes are complex 3D protein molecules created by amino acids‚ forming a unique sequence that produces hydrogen bonds‚ eventually formulating an enzyme within plants and animals (Boyle & Senior‚ 2002). Working alongside other molecules‚ they uphold a stable reaction system. The function of an enzyme is to aid and increase chemical reactions and organise metabolism‚ while maintaining homeostasis
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The Effects of Temperature on the Action of Diastase on a Starch Suspension Hypothesis: The practical being carried out is to observe the effects of temperature on starch break down using a synthesized version of salivary amylase‚ this being Diastase. The starch will be placed into the Diastase and water and then placed in baths of water of different l. temperatures. The test tube containing water will have little or no reaction at all. However‚ the test tube containing the Diastase
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milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering the activation energy required to start a reaction. Enzymes are specific in
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M‚ and 0.2 M sucrose‚ but forgot to label them. After realizing the error‚ the assistant randomly labeled the flasks containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion and osmosis‚ that the assistant could use to determine which of the flasks contains each of the four unknown solutions. Include in your answer: a. a description of how you would set up and perform the experiment; b. the results you would
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
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