of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of two or more substances between
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BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site
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The objective of this lab was to recreate the color profile of a given solution. In this case‚ the solution was Powerade. The final solution should match the absorbance values at the peak wavelengths (420nm and 628nm) in Powerade. This lab was done using deionized water‚ FD&C Blue #1‚ FD&C Yellow #5‚ FD&C Red #40‚ and a spectrometer. To obtain the correct color profile‚ FD&C Blue #1 and FD&C Yellow #5 were utilized in the sample solutions. The experiment was conducted over two days; the first day
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Catalase Lab Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials‚ showing the presence or absence of catalase as observed by the presences of bubbles. Hypothesis: If we pour H2O2 on certain substances‚ the catalase will break down and bubbles will appear at the surface. Data table: Name | Time | Observation | Raw Potato | 1 min. 30 sec. | Slow reaction‚ barley any bubbles. | Raw Liver | 5 min. 30 sec. | Extremely fast reaction‚ very foamy‚ Big
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Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The chemical reaction is shown as 2H2O2 = 2H2O + O2 (Keilin and Hartree 397). The reaction involves primarily the adsorption of hydrogen peroxide at the catalase surface. The decomposition of hydrogen peroxide by catalase is regarded as involving
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CHM151LL Laboratory Report Form Fall 2011 Title: Project 5: SPECTROPHOTOMETRIC ANALYSIS OF A PENNY Date Run: 04/03/2012 Date Submitted: 04/15/2012 Grade: _____________________ Objectives: The purpose of this experiment is to use spectrophotometry to determine the percentage of copper in a copper-clad penny‚ and to determine the thickness of copper layer on the copper-clad penny. Introduction: In the spectrophotometric analysis of a penny lab‚ we will be conducting three
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Non-linear springy objects (rubber bands) Two rectangular weights of ~0.5 kg each to change the mass of the system Procedure: The lab experiment was done in two parts. Part 1 of the experiment was done by 2 different methods. Method 1 was with a force sensor‚ springs‚ and a weight hanger in which masses of 100 gram increments were added (up to 600 grams). A force vs. displacement graph was created and the slope of the graph was recorded. By method 2 the force sensor was tare with a 1 kilogram weight
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selectively permeable membrane and passive processes of simple and facilitated diffusion. The plasma membrane is a gateway which allows nutrients to enter the cell and keep undesirable substances out‚ hence‚ making it selectively permeable. One method of transport is called active transport‚ which uses ATP to transport substances through the membrane. The other is called passive process‚ which does not require ATP energy and the transport process is driven by concentration or pressure differences between
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technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple homogeneous solutions are made. There was not a way to determine the differences in concentrations‚ but the Spec 20 made it possible to measure the difference. The Beer-Lambert Law is a graph used to record
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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