Cain‚ M.L.‚ Wasserman‚ S.A.‚ Minorsky‚ P.V.‚ Jackson‚ R.B. 2008. Biology‚ 8th ed. Pearson Benjamin Cummings‚ San Francisco CA‚ pp. 125-138. Repetto‚ G.‚ del Peso‚ A.‚ LZurita‚ J.2008. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols. 3: 1125 - 1131. Rikhvanov‚ E.G.‚ Varakina‚ N.N.‚ Rusaleva‚ T.M.‚ Rachenko‚ E.I.‚ Voinkov‚ V.K. 2002. The effects of sodium azide on the thermotolerance of the yeasts Saccharomyces cerevisiae and Candida albicans. Microbiology
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Hashimoto Thyroiditis I did not know too much about Hashimoto thyroiditis and I wanted to learn about something that I didn’t know a whole lot about. I knew basic things like patients have to take their medication‚ see their doctor‚ and if a female patient gets pregnant to see their doctor immediately. Hashimoto’s disease is a disease known by many names. They are chronic lymphocytic thyroiditis‚ Hashimoto thyroiditis‚ and autoimmune thyroiditis. It was first discovered by a scientist named Hakaru
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Identification and characterization of Pierisin gene in P. napi The strategy was followed to amplify the Pierisin-6 gene was given in the supplementary Fig. 1. The mRNA of pierisin-6 gene was purified from the total RNA of fifth instar larvae of P. napi‚ the conserved region was amplified using gene specific primers (supplementary table-1)‚ cloned into pGEM®-T vector and sequenced. Further‚ 3’ and 5’ untranslated regions of pierisin-6 gene were identified by 3’/5’ RACE-PCR. The amplified cDNA sequence
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Abstract: Cajanus cajan (L.) is one of the Pigeon pea cultivated in tropical and subtropical areas. It contains many fractions characterized by their bioactivity and efficacy to enhance biological activities. The aim of the present study was to fingerprinting the Cajanus cajan plant ‚ then extract the flavonoids fraction (FFCC) with methanol extract‚ and assess the antimutagenic efficacy of the fraction extract to reduce genotoxicity induced by cyclophosphamide (CP) using chromosome aberrations‚
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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This paper is the first to indicate antiemetic activity of the methanolic extract compositions of A. julibrissin flowers and its protective effect on mitochondria disruption induced by retching in brain of the young chickens. Nausea and vomiting are the symptoms of drug side effects‚ different disease and defenses responsive against poisoning. Recently‚ extensive researches have led to use of medicinal plants as a safe and reliable source of drugs with low or lack of toxicity and side effects [22
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TABLE OF CONTENTS Chapter 1 THE PROBLEM Background of the Study ……………........................................................ Statement of the Problem………..……....................................................... Research Hypothesis…………………....................................................... Significance of the Study …………............................................................ Scope and Delimitation of the Study…........................................................ Definition
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triterpenes and flavonoids‚ which can be a source of medicinal natural products. The species in which the largest number of compounds has been identified is Mussaenda pubescens. Some studies have described lesser known plants of the genus based on its cytotoxicity‚ anti-inflammatory‚ antiviral‚ antioxidant and antibacterial
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Food Chemistry Food Chemistry 95 (2006) 180–185 www.elsevier.com/locate/foodchem Antioxidant activity and hepatoprotective potential of Phyllanthus niruri R. Harish‚ T. Shivanandappa * Department of Food Protectants and Infestation Control‚ Central Food Technological Research Institute‚ Mysore 570 020‚ India Received 5 April 2004; received in revised form 25 November 2004; accepted 25 November 2004 Abstract Antioxidant activity and hepatoprotective potential of Phyllanthus niruri
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activity of Super oxide dismutase and reduced glutathion content. Dual staining with ethidium bromide and propidium Iodide staining revealed that HepG2 cells treated with A.catechu seed extract caused cell death mainly by apoptosis. The events of cytotoxicity and oxidative stress were found to be associated with up-regulation of pro-apoptotic gene expressions such as bax‚ Cyt-c‚ caspase-3 and 9‚ and down-regulation of anti-apoptotic marker bcl-2. In addition‚ there was a significant increase of sub-
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