G-protein Coupled Receptors (GPCRs) are cell surface proteins that relay extracellular signals to the nucleus. These signals can be in the form of light‚ peptides‚ lipids‚ and sugars. Once activated by a ligand at the outer cell surface‚ GPCRs activate their cognate G-proteins that reside on the inner cell membrane. The activated G-proteins then slide along the inside of the cell membrane to activate a cascade of proteins and enzymes that in turn amplify the original message received by the GPCR
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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The Sri-Lankan Construction Industry in the New Millennium C WEDDIKKARA Dept. of Management‚ Construction and Building Science University of Western Sydney‚ Australia d.weddikkara@uws.edu.au K. DEVAPRIYA Dept. of Building Economics University of Moratuwa‚ Sri Lanka (Research Scholar‚ University of Hong Kong) Abstract The construction investment in Sri- Lanka has followed the economic changes that took place during the last decade. This resulted in a significant change particularly in the supply
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Introduction Over the past decade‚ medical costs have increased more rapidly than other consumer costs. Americans spent 2.5 trillion on health care in 2009 according to Medicare’s Office of the Actuary. That figure translates into approximately $8‚086 per person‚ or 17.6 percent of the nation’s gross domestic product (GDP).1 Health care costs more than tripled from 1990 to 20092 and are projected to rise to 19.6 percent of GDP in 2019.3 “The 4 percent increase from 2008 levels represented
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Self-Determination Theory – How Do You Feel? PSYC3520 - Intro to Social Psychology Social Psychological Theory April‚ 2014 What is the Self-Determination Theory? “Self-determination theory (SDT) is a macro theory of human motivation and personality‚ concerning people ’s inherent growth tendencies and their innate psychological needs.” (Deci & Ryan‚ n.d.) In short‚ it is our motivation in the choices we make that exclude outside influences. This theory of motivation
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Experiment A Spectroscopic determination of Iron with Phenanthroline Unknown # 2 Mass of Mohr’s Salt: 0.2040 1. 5mL of 6M acetic acid was measured and transferred into a 100mL volumetric flask with a pipette and diluted to the mark. Concentration = [0.2040(±0.0001)g/100(±0.08)mL]*[(1mol/392.16g)/(1L/1000mL)] = 0.005202(±0.09382%) = 0.005202(±0.000005)mol/L 2. 10mL of the above stock solution was transferred to a 250 mL volumetric flask and diluted to the mark. Concentration
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have for protein synthesis? Explanation: The mRNA encodes the amino acid sequence of a protein. During the translation‚ ribosomal RNA combines with other proteins to form a ribosome which amino acids are transported to the ribosome. The combination of mRNA and tRNA converts the mRNA into the amino acid sequence of the protein. Did the two mutations result in a change in the final proteins? If so‚ describe the change. Response/ explanation: A change took place in the final proteins because
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The first half of module seven discussed protein digestion‚ absorption‚ and metabolism; while the second half focused on pathological stress and kidney disease. Protein digestion occurs in the stomach and small intestine. When a protein reaches the stomach it is denatured by hydrochloric acid. Once the protein is digested pepsin breaks apart the denatured protein into peptides. Peptides move into the small intestine where they encounter an enzyme that digest the peptides into di- or tripeptides‚
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BUSINESS STANDARD A PROJECT REPORT ON MARKET PENETRATION STRATEGY & SURVEY OF POTENTIAL CONSUMER PROJECT GUIDE -: MR. KUNAL PANCHAL FOR THE PARTIAL FULFILLMENT OF MASTER IN BUSINESS ADMINISTRATION SUBMITTED BY: ANAND SINGH (99) RAGHUVIRSINH SOLANKI (101) SUBMITTED TO S.K.PATEL INSTITUTE OF MANAGEMENT AND COMPUTER STUDIES. (S.K.P.I.M.C.S.) GANDHINAGAR‚ INDIA JULY-2006 1 ACKNOWLEDGEMENT Writing a dissertation can never be the work of any one person. It includes co-operation & efforts
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Construction Economics Assignment 01 B. Anushka Priyamal CSCT2014498 1 Acknowledgement Firstly‚ I would like to offer my special thanks to our Construction Economics lecture Dr. Nirodha Fernando who gave the knowledge about Construction Economics subject and given advice and guide to finish this assignment. I would also like to offer thank for the staff of the CSCT campus and CSCT librarians who gave books and lab facilities in this attempt. Finally‚ my heartfelt thanks are also offered my parents
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