Spectrophotometric Determination of Iron in Aqueous Solutions as a Complex of 1‚10-Phenanthroline Introduction From the previous classical method of concentration determination of an unknown sample‚ this experiment now deals with the instrumental one. Unknown concentrations of iron in solutions were determined by measuring their corresponding absorbances through spectrophotometry. A spectrophotometer measures the amount of light that a solution absorbs. This is done by bombarding the sample with a
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Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table 2: Experimental
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Investigation - Solution Colour and Ion Concentration Aim: To investigate whether solution colour can be used to reliably determine the concentration of coloured ions in a solution. Hypothesis: the concentration of permanganate ions in the solution is inversely proportional to the percentage transmission of light through the solution. Dependent variable: concentration of permanganate ions. Independent variable: percentage transmission of light through the solution. Equipment: 20 ml
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Practical report: Determination of concentration using a standard curve Introduction Spectrophotometer is an instrument that confirms the measurement of specified wavelength of light that passes through medium. The measurement of light absorbance by a solution is also done with spectrophometer. Absorption spectrum Spectrometers are mainly and widely functional in identifying the components of solutions that helps determine the components’ concentrations. Compounds are experimentally identified
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the molecules may have been moving slower. Part B: Title: The effect of salt concentration on osmosis in potato. Introduction: 1. The purpose of doing this lab is to observe the effects of various solute concentrations on osmosis in a potato. 2. During osmosis‚ water moves from the side of the membrane where they are most concentrated to the side where they are less concentrated. If the concentration is equal on both sides‚ there will be no movement. 3. I hypothesize that
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In this folio task‚ the impact of drug absorption and blood stream concentration‚ on the human body‚ will be observed. The rate of absorption and clearance of the drug‚ relies on many factors. The drugs blood stream concentration‚ time of administration‚ absorption rate‚ clearance rate and the effect of certain parameters‚ will be calculated. This can be completed through using a surge function‚ calculus (derivatives) and straight line graphs (). Graphing technology on the graphics calculator
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Noel Arackal 1.25.16 Bisc 4930 Experiment No. 1 – Varying Extracellular Concentration Introduction: This experiment sets out to determine the tonicity of solutions with varying concentrations of sodium chloride on the red blood cells of sheep by measuring the transmittance of a red blood cell/NaCl solution with a spectrometer. This is done in order to study the effects of solutions containing varying levels of tonicity to red blood cells. Tonicity describes what happens to a cell when it is
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For this data analysis project‚ I conducted and experiment to investigate how the concentration of an acid affects the rate of the reaction. I have set up the experiment’s equipment as shown on figure 1. As you can see by figure 1‚ I have used the reaction between sodium thisoulphate solution and hydrochloric acid (HCL) to come up with results that will help me draw conclusions regarding the effects of the concentration of the acid. I decided to use this particular reaction due to the fact that the
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investigation: The effect of solute concentration on osmosis in potatoes. Research question: What is the solute concentration of a potato? Hypothesis: Osmosis is the movement of water across the membrane from an area of high concentration to an area of low concentration. The osmosis continues until the solute concentrations are equal in both areas. In this experiment‚ we put pieces of potatoes into test tubes with sugar solutions of different concentrations and leave them there over night. It
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The Visible Spectra Of Orange Soda Pop Objective : To measure an absorbance value of the different colour solutions by using Spectrophotometry Introduction Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color‚ or more specifically‚ the wavelength of light (nm). The instrument also can be use on biological sample such as chlorophyll pigments and
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