Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning
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Gabriel Alizaidy Liquid Chromatography September 14‚ 2009 Objective: To separate the components of unsweetened‚ grape flavored Kool-Aid. Procedure: Using two syringes‚ inject different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
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